| Overview |
| bs-10180r-rbitc-100ul |
| Adeno-Associated Virus Capsid Protein VP1 Antibody, RBITC Conjugated |
| IF(IHC-P), IF(IHC-F), IF(ICC) |
| This antibody will recognize many AAV strains, including AAV5, AAV4, AAV3B, AAV9, and AAV13 |
| Virus, Others |
| Specifications |
| RBITC |
| Rabbit |
| KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1 |
| 21-120/724 |
| Polyclonal |
| IgG |
| 1ug/ul |
| Purified by Protein A. |
| Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target |
| capsid protein Adeno-associated 5 virus; capsid protein [Adeno-associated virus - 5]; capsid protein AAV5; Parvovirus coat protein VP1; capsid protein. |
| Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus |
| Application Dilution |
| IF(IHC-P) |
1:50-200 |
| IF(IHC-F) |
1:50-200 |
| IF(ICC) |
1:50-200 |
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