ACE Polyclonal Antibody

Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-ACE1 Polyclonal Antibody, Unconjugated (bs-0439R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Lane 1: mouse lung lysates Lane 2: mouse liver lysates, Lane 3: mouse adrenal lysates probed with Rabbit Rabbit Anti-ACE Polyclonal Antibody, Unconjugated (bs-0439R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat
Dog

Predicted Reactivity

Cow
Sheep
Pig

Overview
Catalog #	bs-0439R
Product Name	ACE Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat, Dog
Predicted Reactivity	Cow, Sheep, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from Human ACE1
Immunogen Range	801-900/1306
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1636
Swiss Prot	P12821
Subcellular location	Secreted, Cell membrane
Synonyms	Angiotensin Converting Enzyme 1; ACE; ACE-T; Dipeptidyl carboxy peptidase 1; Kininase II; ACE-1; testis-specic isoform precursor; ACE1; Angiotensin converting enzyme testis specic isoform; Carboxycathepsin; CD 143; DCP 1; Dipeptidyl carboxypeptidase 1; MVCD3; Peptidase P; Peptidyl dipeptidase A; Testicular ECA
Background	Angiotensin Converting enzyme is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500