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U937 lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-20351R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Bcl-2 Polyclonal Antibody(bs-20351R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Recombinant human Bcl-2 protein, C-His probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-20351R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-20351R

Applications: WB, ELISA, FCM

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled withAnti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032r) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse embryo lysates, L2 mouse brain lysates probed with Anti- Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. P

Catalog #1: bs-0032R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: Unconjugated

Catalog #1: bs-0032R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 350

Catalog #1: bs-4563R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: AbBy Fluor® 350

Catalog #1: bs-15533R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor® 350

Catalog #1: bs-20351R-BF350

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0032R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 405

Catalog #1: bs-4563R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: AbBy Fluor® 405

Catalog #1: bs-15533R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor® 405

Catalog #1: bs-20351R-BF405

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0032R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 488

Catalog #1: bs-4563R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: AbBy Fluor® 488

Catalog #1: bs-15533R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor® 488

Catalog #1: bs-20351R-BF488

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0032R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 555

Catalog #1: bs-4563R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: AbBy Fluor® 555

Catalog #1: bs-15533R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor® 555

Catalog #1: bs-20351R-BF555

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0032R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 594

Catalog #1: bs-4563R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: AbBy Fluor® 594

Catalog #1: bs-15533R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor® 594

Catalog #1: bs-20351R-BF594

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0032R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 647

Catalog #1: bs-4563R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: AbBy Fluor® 647

Catalog #1: bs-15533R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor® 647

Catalog #1: bs-20351R-BF647

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0032R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 680

Catalog #1: bs-4563R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: AbBy Fluor® 680

Catalog #1: bs-15533R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor® 680

Catalog #1: bs-20351R-BF680

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0032R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 750

Catalog #1: bs-4563R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: AbBy Fluor® 750

Catalog #1: bs-15533R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor® 750

Catalog #1: bs-20351R-BF750

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0032R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: APC

Catalog #1: bs-4563R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: APC

Catalog #1: bs-15533R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: APC

Catalog #1: bs-20351R-APC

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: APC

Catalog #1: bs-0032R-APC-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: APC-Cy5

Catalog #1: bs-4563R-APC-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: APC-Cy5

Catalog #1: bs-15533R-APC-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5

Catalog #1: bs-20351R-APC-Cy5

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: APC-Cy5

Catalog #1: bs-0032R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: APC-Cy5.5

Catalog #1: bs-4563R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: APC-Cy5.5

Catalog #1: bs-15533R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bs-20351R-APC-Cy5.5

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-0032R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: APC-Cy7

Catalog #1: bs-4563R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: APC-Cy7