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Catalog #1: bs-14399R-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-14399R-Cy3

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-14399R-Cy5

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-14399R-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-14399R-Cy7

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-14399R-FITC

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-14399R-HRP

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-14399R-PE

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bs-14399R-PE-Cy3

Applications: WB

Reactivity: Human

Conjugate: PE-Cy3

Catalog #1: bs-14399R-PE-Cy5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bs-14399R-PE-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bs-14399R-PE-Cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bs-14399R-PerCP

Applications: WB

Reactivity: Human

Conjugate: PerCP

Catalog #1: bs-14399R-PerCP-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bs-14399R-PerCP-Cy7

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bs-14399R-RBITC

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bs-14408R

Applications: ELISA, IHC-P, IHC-F, IF, ICC

Reactivity: Plant

Conjugate: Unconjugated

Catalog #1: bs-14408R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Plant

Conjugate: Biotin

Catalog #1: bs-14408R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Plant

Conjugate: HRP

A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-EGFR(S695)) Monoclonal Antibody, Unconjugated (bsm-54568R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-EGFR(S695)) Monoclonal Antibody, Unconjugated (bsm-54568R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-EGFR(S695)) Monoclonal Antibody, Unconjugated (bsm-54568R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Catalog #1: bsm-54568R

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-EGFR(Y1068)) Monoclonal Antibody, Unconjugated (bsm-54587R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-EGFR(Y1068)) Monoclonal Antibody, Unconjugated (bsm-54587R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-EGFR(Y1068)) Monoclonal Antibody, Unconjugated (bsm-54587R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Flow cytometric analysis of Phospho-EGFR(Y1068) was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (Phospho-EGFR(Y1068), 1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Catalog #1: bsm-54587R

Applications: WB, FCM, IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-eIF-2a(S51) Antibody, Unconjugated (bsm-54490R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-eIF-2a(S51) Antibody, Unconjugated (bsm-54490R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-eIF-2a(S51) Antibody, Unconjugated (bsm-54490R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human prostate cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-eIF-2a(S51) Antibody, Unconjugated (bsm-54490R) at 1:50 for 30 minutes at room temperature, DAB staining.

Catalog #1: bsm-54490R

Applications: WB, FCM, IHC-P, IF(ICC), IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60678R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60678R-BF350

Applications: WB

Reactivity:

Conjugate: AbBy Fluor® 350

Catalog #1: bsm-60678R-BF405

Applications: WB

Reactivity:

Conjugate: AbBy Fluor® 405

Catalog #1: bsm-60678R-BF488

Applications: WB

Reactivity:

Conjugate: AbBy Fluor® 488

Catalog #1: bsm-60678R-BF555

Applications: WB

Reactivity:

Conjugate: AbBy Fluor® 555

Catalog #1: bsm-60678R-BF594

Applications: WB

Reactivity:

Conjugate: AbBy Fluor® 594

Catalog #1: bsm-60678R-BF647

Applications: WB

Reactivity:

Conjugate: AbBy Fluor® 647

Catalog #1: bsm-60678R-BF680

Applications: WB

Reactivity:

Conjugate: AbBy Fluor® 680

Catalog #1: bsm-60678R-BF750

Applications: WB

Reactivity:

Conjugate: AbBy Fluor® 750

Catalog #1: bsm-60678R-APC

Applications: WB

Reactivity:

Conjugate: APC

Catalog #1: bsm-60678R-APC-Cy5

Applications: WB

Reactivity:

Conjugate: APC-Cy5

Catalog #1: bsm-60678R-APC-Cy5.5

Applications: WB

Reactivity:

Conjugate: APC-Cy5.5

Catalog #1: bsm-60678R-APC-Cy7

Applications: WB

Reactivity:

Conjugate: APC-Cy7

Catalog #1: bsm-60678R-Biotin

Applications: WB

Reactivity:

Conjugate: Biotin

Catalog #1: bsm-60678R-Cy3

Applications: WB

Reactivity:

Conjugate: Cy3

Catalog #1: bsm-60678R-Cy5

Applications: WB

Reactivity:

Conjugate: Cy5

Catalog #1: bsm-60678R-Cy5.5

Applications: WB

Reactivity:

Conjugate: Cy5.5

Catalog #1: bsm-60678R-Cy7

Applications: WB

Reactivity:

Conjugate: Cy7

Catalog #1: bsm-60678R-FITC

Applications: WB

Reactivity:

Conjugate: FITC

Catalog #1: bsm-60678R-HRP

Applications: WB

Reactivity:

Conjugate: HRP

Catalog #1: bsm-60678R-PE

Applications: WB

Reactivity:

Conjugate: PE

Catalog #1: bsm-60678R-PE-Cy3

Applications: WB

Reactivity:

Conjugate: PE-Cy3

Catalog #1: bsm-60678R-PE-Cy5

Applications: WB

Reactivity:

Conjugate: PE-Cy5

Catalog #1: bsm-60678R-PE-Cy5.5

Applications: WB

Reactivity:

Conjugate: PE-Cy5.5

Catalog #1: bsm-60678R-PE-Cy7

Applications: WB

Reactivity:

Conjugate: PE-Cy7

Catalog #1: bsm-60678R-PerCP

Applications: WB

Reactivity:

Conjugate: PerCP