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Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-14-3-3 Polyclonal Antibody (bs-0237R, Unconjugated) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) at 1:200 for 40 minutes at 37\u00b0C
L1 mouse brain lysates L2 mouse kidney lysates probed with Anti 14-3-3(Alpha\/Beta\/Gamma\/Delta\/Epsilon) Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 31kD. Observed band size: 31kD.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0237R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Yeast

Conjugate: Unconjugated

Catalog #1: bsm-61711r-100ul

Applications: WB

Reactivity: Yeast

Conjugate: Unconjugated

Catalog #1: bs-8783R

Applications: WB

Reactivity: Yeast

Conjugate: Unconjugated

Catalog #1: bs-2950R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: Unconjugated

Catalog #1: bs-2950R-BF350

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: AbBy Fluor® 350

Catalog #1: bs-2950R-BF405

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: AbBy Fluor® 405

Catalog #1: bs-2950R-BF488

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: AbBy Fluor® 488

Catalog #1: bs-2950R-BF555

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: AbBy Fluor® 555

Catalog #1: bs-2950R-BF594

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: AbBy Fluor® 594

Catalog #1: bs-2950R-BF647

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: AbBy Fluor® 647

Catalog #1: bs-2950R-BF680

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: AbBy Fluor® 680

Catalog #1: bs-2950R-BF750

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: AbBy Fluor® 750

Catalog #1: bs-2950R-APC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: APC

Catalog #1: bs-2950R-APC-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: APC-Cy5.5

Catalog #1: bs-2950R-APC-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: APC-Cy7

Catalog #1: bs-2950R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Yeast

Conjugate: Biotin

Catalog #1: bs-2950R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: Cy3

Catalog #1: bs-2950R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: Cy5

Catalog #1: bs-2950R-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: Cy5.5

Catalog #1: bs-2950R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: Cy7

Catalog #1: bs-2950R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: FITC

Catalog #1: bs-2950R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Yeast

Conjugate: HRP

Catalog #1: bs-2950R-PerCP

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: PerCP

Catalog #1: bs-2950R-PerCP-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: PerCP-Cy5.5

Catalog #1: bs-2950R-RBITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Yeast

Conjugate: RBITC

Lane 1: Yeast lysates probed with GAPDH (Yeast) Polyclonal Antibody, Unconjugated (bs-8789R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Yeast lysates probed with GAPDH Polyclonal Antibody, Unconjugated (bs-8789R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-8789R

Applications: WB

Reactivity: Yeast

Conjugate: Unconjugated

Catalog #1: bs-8789R-BF350

Applications: WB

Reactivity: Yeast

Conjugate: AbBy Fluor® 350

Catalog #1: bs-8789R-BF405

Applications: WB

Reactivity: Yeast

Conjugate: AbBy Fluor® 405

Catalog #1: bs-8789R-BF488

Applications: WB

Reactivity: Yeast

Conjugate: AbBy Fluor® 488

Catalog #1: bs-8789R-BF555

Applications: WB

Reactivity: Yeast

Conjugate: AbBy Fluor® 555

Catalog #1: bs-8789R-BF594

Applications: WB

Reactivity: Yeast

Conjugate: AbBy Fluor® 594

Catalog #1: bs-8789R-BF647

Applications: WB

Reactivity: Yeast

Conjugate: AbBy Fluor® 647

Catalog #1: bs-8789R-BF680

Applications: WB

Reactivity: Yeast

Conjugate: AbBy Fluor® 680

Catalog #1: bs-8789R-BF750

Applications: WB

Reactivity: Yeast

Conjugate: AbBy Fluor® 750

Catalog #1: bs-8789R-APC

Applications: WB

Reactivity: Yeast

Conjugate: APC

Catalog #1: bs-8789R-APC-Cy5.5

Applications: WB

Reactivity: Yeast

Conjugate: APC-Cy5.5

Catalog #1: bs-8789R-APC-Cy7

Applications: WB

Reactivity: Yeast

Conjugate: APC-Cy7

Catalog #1: bs-8789R-Biotin

Applications: WB

Reactivity: Yeast

Conjugate: Biotin

Catalog #1: bs-8789R-Cy3

Applications: WB

Reactivity: Yeast

Conjugate: Cy3

Catalog #1: bs-8789R-Cy5

Applications: WB

Reactivity: Yeast

Conjugate: Cy5

Catalog #1: bs-8789R-Cy5.5

Applications: WB

Reactivity: Yeast

Conjugate: Cy5.5

Catalog #1: bs-8789R-Cy7

Applications: WB

Reactivity: Yeast

Conjugate: Cy7

Catalog #1: bs-8789R-FITC

Applications: WB

Reactivity: Yeast

Conjugate: FITC

Catalog #1: bs-8789R-HRP

Applications: WB

Reactivity: Yeast

Conjugate: HRP

Catalog #1: bs-8789R-PE

Applications: WB

Reactivity: Yeast

Conjugate: PE

Catalog #1: bs-8789R-PE-Cy5

Applications: WB

Reactivity: Yeast

Conjugate: PE-Cy5

Catalog #1: bs-8789R-PE-Cy5.5

Applications: WB

Reactivity: Yeast

Conjugate: PE-Cy5.5

Catalog #1: bs-8789R-PE-Cy7

Applications: WB

Reactivity: Yeast

Conjugate: PE-Cy7