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Catalog #1: bs-0741r-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: PE-Cy7

Catalog #1: bs-0741r-percp-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: PerCP

Catalog #1: bs-0741r-percp-cy55-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0741r-rbitc-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: RBITC

Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN1A\/p21 Polyclonal Antibody, Unconjugated (bs-22795R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN1A\/p21 Polyclonal Antibody, Unconjugated (bs-22795R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-22795R

Applications: IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-22795R-Biotin

Applications: IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-22795R-HRP

Applications: IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Mouse muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN1B (Ser178) Polyclonal Antibody, Unconjugated (bs-5236R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN1B (Ser178) Polyclonal Antibody, Unconjugated (bs-5236R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Rat Spleen lysates; Lane 3: Rat Thymus lysates; Lane 4: Human Hela cell lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human THP-1 cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human MCF-7 cell lysates probed with CDKN1B Polyclonal Antibody, Unconjugated (bs-5236R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5236R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

MCF-7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CDKN1B (Thr157) Polyclonal Antibody(bs-5228R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN1B (Thr157) Polyclonal Antibody, Unconjugated (bs-5228R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN1B (Thr157) Polyclonal Antibody, Unconjugated (bs-5228R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Spleen lysates; Lane 2: Mouse Thymus lysates; Lane 3: Rat Thymus lysates probed with CDKN1B Polyclonal Antibody, Unconjugated (bs-5228R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5228R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN1B (Thr198) Polyclonal Antibody, Unconjugated (bs-5279R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN1B (Thr198) Polyclonal Antibody, Unconjugated (bs-5279R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN1B (Thr198) Polyclonal Antibody, Unconjugated (bs-5279R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5279R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN1B (Ser10) Polyclonal Antibody, Unconjugated (bs-20074R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN1B (Ser10) Polyclonal Antibody, Unconjugated (bs-20074R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-20074R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-20074R-BF350

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-20074R-BF405

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-20074R-BF488

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-20074R-BF555

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-20074R-BF594

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bs-20074R-BF647

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bs-20074R-BF680

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 680

Catalog #1: bs-20074R-BF750

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 750

Catalog #1: bs-20074R-APC

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-20074R-APC-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5

Catalog #1: bs-20074R-APC-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-20074R-APC-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-20074R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-20074R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-20074R-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-20074R-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-20074R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-20074R-FITC

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-20074R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-20074R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-20074R-PE-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy3

Catalog #1: bs-20074R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-20074R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-20074R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-20074R-PerCP

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-20074R-PerCP-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-20074R-PerCP-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bs-20074R-RBITC

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Formalin-fixed and paraffin embedded human ovary carcinoma labeled Anti-CDKN1B\/P27kip1 Polyclonal Antibody, Unconjugated (bs-0742R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
RSC96 probed with CDKN1B Polyclonal Antibody, Unconjugated (bs-0742R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
MCF-7 cells were stained with CDKN1B Polyclonal Antibody, Unconjugated(bs-0742R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Mouse lung lysates probed with CDKN1B Polyclonal Antibody, unconjugated (bs-0742R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-0742R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Others

Conjugate: Unconjugated

Catalog #1: bs-0742r-bf350-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Others

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0742r-bf405-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Others

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0742r-bf488-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Others

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0742r-bf555-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Others

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0742r-bf594-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Others

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0742r-bf647-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Others

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0742r-bf680-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Others

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0742r-bf750-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Others

Conjugate: AbBy Fluor® 750