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Catalog #1: bs-7357R-PE-Cy3

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy3

Catalog #1: bs-7357R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-7357R-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-7357R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-7357R-PerCP

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-7357R-PerCP-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-7357R-PerCP-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bs-7357R-RBITC

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bs-17226R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-17226R-BF350

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-17226R-BF405

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-17226R-BF488

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-17226R-BF555

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-17226R-BF594

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bs-17226R-BF647

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bs-17226R-BF680

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 680

Catalog #1: bs-17226R-BF750

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 750

Catalog #1: bs-17226R-APC

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-17226R-APC-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5

Catalog #1: bs-17226R-APC-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-17226R-APC-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-17226R-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-17226R-Cy3

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-17226R-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-17226R-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-17226R-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-17226R-FITC

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-17226R-HRP

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-17226R-PE

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-17226R-PE-Cy3

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy3

Catalog #1: bs-17226R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-17226R-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-17226R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-17226R-PerCP

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-17226R-PerCP-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-17226R-PerCP-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bs-17226R-RBITC

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: room temperature 2h\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: 293, 3: A20, 4: Rat brain\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 38 kDa\r\nObserved MW: 38 kDa
Tissue: Human liver cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60463M
Tissue: Rat kidney\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60463M

Catalog #1: bsm-60463M

Applications: WB, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HepG2 cells with Topoisomerase I (1D1) Monoclonal Antibody (bsm-52531R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: HepG2 Cells; Lane 2: Jurkat Cells; Lane 3: MCF-7 Cells; Probed with Topoisomerase I (1D1) Monoclonal Antibody (bsm-52531R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Topoisomerase I (1D1) Monoclonal Antibody (bsm-52531R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Topoisomerase I (1D1) Monoclonal Antibody (bsm-52531R) at 1:300 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52531R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Human LO2 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human SW480 cell lysates probed with Topoisomerase I Polyclonal Antibody, Unconjugated (bs-23144R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Topoisomerase I) Polyclonal Antibody, Unconjugated (bs-23144R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Topoisomerase I) Polyclonal Antibody, Unconjugated (bs-23144R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Topoisomerase I) Polyclonal Antibody, Unconjugated (bs-23144R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-23144R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-23144R-BF350

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-23144R-BF405

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-23144R-BF488

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-23144R-BF555

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-23144R-BF594

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bs-23144R-BF647

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bs-23144R-BF680

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 680

Catalog #1: bs-23144R-BF750

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 750