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Formalin-fixed and paraffin embedded rat testis labeled with Anti-Acrosin Polyclonal Antibody, Unconjugated (bs-5151R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HUVEC cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Acrosin Polyclonal Antibody(bs-5151R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Acrosin) polyclonal Antibody, Unconjugated (bs-5151R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Acrosin Polyclonal Antibody(bs-5151R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-5151R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Unconjugated

Catalog #1: bs-5151R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-5151R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-5151R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-5151R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-5151R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: AbBy Fluor® 594

Catalog #1: bs-5151R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: AbBy Fluor® 647

Catalog #1: bs-5151R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: AbBy Fluor® 680

Catalog #1: bs-5151R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: AbBy Fluor® 750

Catalog #1: bs-5151R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: APC

Catalog #1: bs-5151R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: APC-Cy5.5

Catalog #1: bs-5151R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: APC-Cy7

Catalog #1: bs-5151R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Biotin

Catalog #1: bs-5151R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy3

Catalog #1: bs-5151R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy5

Catalog #1: bs-5151R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy5.5

Catalog #1: bs-5151R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy7

Catalog #1: bs-5151R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: FITC

Catalog #1: bs-5151R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Goat

Conjugate: HRP

Catalog #1: bs-5151R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Goat

Conjugate: PE

Catalog #1: bs-5151R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Goat

Conjugate: PE-Cy5

Catalog #1: bs-5151R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Goat

Conjugate: PE-Cy5.5

Catalog #1: bs-5151R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Goat

Conjugate: PE-Cy7

Catalog #1: bs-5151R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: PerCP

Catalog #1: bs-5151R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-5151R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: RBITC

Catalog #1: bs-0118r-100ul

Applications: IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: Unconjugated

Catalog #1: bs-0118r-bf350-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0118r-bf405-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0118r-bf488-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0118r-bf555-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0118r-bf594-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0118r-bf647-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0118r-bf680-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0118r-bf750-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0118r-apc-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: APC

Catalog #1: bs-0118r-apc-cy5-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: APC-Cy5

Catalog #1: bs-0118r-apc-cy55-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: APC-Cy5.5

Catalog #1: bs-0118r-apc-cy7-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: APC-Cy7

Catalog #1: bs-0118r-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: Biotin

Catalog #1: bs-0118r-cy3-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: Cy3

Catalog #1: bs-0118r-cy5-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: Cy5

Catalog #1: bs-0118r-cy55-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: Cy5.5

Catalog #1: bs-0118r-cy7-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: Cy7

Catalog #1: bs-0118r-fitc-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: FITC

Catalog #1: bs-0118r-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: HRP

Catalog #1: bs-0118r-pe-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: PE

Catalog #1: bs-0118r-percp-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: PerCP