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Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-ATM (Tyr170) Antibody(bs-11707R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-ATM (Ser794) Antibody(bs-11707R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-11707R

Applications: IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-ATM Polyclonal Antibody, Unconjugated (bs-1370R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded human cervical cancer labeled with Anti-ATM Polyclonal Antibody, Unconjugated (bs-1370R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-ATM Polyclonal Antibody (bs-1370R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Hela cells probed with ATM Polyclonal Antibody, Unconjugated (bs-1370R) at 3ug for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1370R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2272R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Chicken

Conjugate: Unconjugated

Catalog #1: bs-34235R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse kidney; Lane 2: Mouse colon; Lane 3: MCF-7 cells; Probed with ATP citRate lyase (3G8) Monoclonal Antibody (bsm-52458R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with ATP citRate lyase (3G8) Monoclonal Antibody (bsm-52458R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ATP citRate lyase (3G8) Monoclonal Antibody (bsm-52458R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with ATP citRate lyase (3G8) Monoclonal Antibody (bsm-52458R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52458R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3037R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3929R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-4065R

Applications: WB, ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9885R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3035R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3064R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3034R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-9570R

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Anti-ATP1A1 Polyclonal Antibody, Unconjugated (bs-4255R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Lovo cell lysates; Lane 2: Mouse Cerebrum lysates probed with ATP1A1 Polyclonal Antibody, Unconjugated (bs-4255R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4255R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ATP1A1 ) Polyclonal Antibody, Unconjugated (bs-42166R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ATP1A1 ) Polyclonal Antibody, Unconjugated (bs-42166R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ATP1A1 ) Polyclonal Antibody, Unconjugated (bs-42166R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ATP1A1 ) Polyclonal Antibody, Unconjugated (bs-42166R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-42166R

Applications: IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse colon labeled with Anti- ATP1b2\/Na+K+ATPase Polyclonal Antibody, Unconjugated (bs-1152R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates L2 rat colon lysates probed with Anti ATP1b2\/Na+K+ATPase Polyclonal Antibody, Unconjugated (bs-1152R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 33kD. Observed band size: 33kD.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Human K562 cell lysates; Lane 3: Human SH-SY5Y cell lysates probed with ATPase Na+\/ K+ beta 2 Polyclonal Antibody, Unconjugated (bs-1152R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1152R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human Fetal Skeletal Muscle; Probed with ATP2A1 (3G1) Monoclonal Antibody (bsm-54466R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with ATP2A1 (3G1) Monoclonal Antibody (bsm-54466R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Catalog #1: bsm-54466R

Applications: WB, IHC-P, IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2434R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2433R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60257M

Applications: WB, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: HepG2, 4T1, SH-SY5Y, BRL\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 3 s\r\nPredicted MW: 50 kDa\r\nObserved MW: 50 kDa
Tissue: Rat kidney\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60482M
Tissue: Mouse heart\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60482M
Tissue: Human skeletal muscle\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60482M

Catalog #1: bsm-60482M

Applications: WB, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of Hela cells with ATP5A1 (7C1) Monoclonal Antibody (bsm-52928R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black)
Lane 1: Hela cells lysates; Lane 2: MCF-7 cells lysates; Probed with ATP5A1 (7C1) Monoclonal Antibody (bsm-52928R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with ATP5A1 (7C1) Monoclonal Antibody (bsm-52928R) at 1:200 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart muscle tissue incubated with ATP5A1 (7C1) Monoclonal Antibody (bsm-52928R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52928R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8600R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Rat, Pig, Sheep

Conjugate: Unconjugated

Catalog #1: bs-12547R

Applications: ELISA, IHC-F

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60380M

Applications: WB, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-7691R

Applications: ELISA, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12548R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-12549R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-10930R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with ATP7A Polyclonal Antibody, Unconjugated (bs-1572R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with ATP7A Polyclonal Antibody (bs-1572R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-1572R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-ATP7B Polyclonal Antibody, Unconjugated (bs-1718R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ATP7B Polyclonal Antibody, Unconjugated (bs-1718R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ATP7B Polyclonal Antibody, Unconjugated (bs-1718R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ATP7B) Polyclonal Antibody, Unconjugated (bs-1718R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1718R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-12550R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse bone lysates; Lane 2:Mouse lung lysates probed with ATPase Na+\/ K+ beta 2 Polyclonal Antibody, Unconjugated (bs-23414R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with ATPase Na+\/ K+ beta 2 Polyclonal Antibody, Unconjugated (bs-23414R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-23414R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nLysate: HeLa, N2a, BRL, Mouse heart\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 52 kDa\r\nObserved MW: 52 kDa
Tissue: Human breast cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60217M
Tissue: Mouse kidney\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60217M

Catalog #1: bsm-60217M

Applications: WB, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cells lysates; Lane 2: HepG2 cells lysates; Line 3: 293T cells lysates; Probed with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52904R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Catalog #1: bs-75082c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60260M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51642M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-70024R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-11224R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11223R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1528R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-11504R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-71157R

Applications: ELISA/RIA

Reactivity: Animal

Conjugate: Unconjugated

Catalog #1: bs-24302R

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12551R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-7974R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-3008R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Rat

Conjugate: Unconjugated