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Catalog #1: bs-2911R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Flow cytometric analysis of K562 cells with MEK7 (7C6) Monoclonal Antibody (bsm-52666R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1:Hela Cells; Lane 2: 293T Cells; Lane 3: A431 Cells; Probed with MEK7 (7C6) Monoclonal Antibody (bsm-52666R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with MEK7 (7C6) Monoclonal Antibody (bsm-52666R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with MEK7 (7C6) Monoclonal Antibody (bsm-52666R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52666R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HepG2 cells with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: HepG2; Lane 2: Rat brain; Lane 3: SW480 Cells; Probed with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52540R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with MEKK3 (5C1) Monoclonal Antibody (bsm-52709R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela Cells; Lane 2: 293T Cells; Lane 3: A431 Cells; Probed with MEKK3 (5C1) Monoclonal Antibody (bsm-54089R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with MEKK3 (5C1) Monoclonal Antibody (bsm-52709R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with MEKK3 (5C1) Monoclonal Antibody (bsm-52709R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52709R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9673R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-71146R

Applications: ELISA/RIA

Reactivity: Animal

Conjugate: Unconjugated

Catalog #1: bsm-2182M

Applications: ELISA, IHC-P, IF(IHC-P)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-7362R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-9665R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-21739R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded rat brain labeled with Rabbit Anti-MTNR1A\/MTR-1A\/MEL-1A-R Polyclonal Antibody, Unconjugated (bs-0027R) at 1:300 followed by conjugation to the secondary antibody
Lane 1: Cerebrum lysates probed with Melatonin Receptor 1A Antibody (bs-0027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
SH-SY5Y cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Melatonin Receptor 1A Polyclonal Antibody(bs-0027R) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.

Catalog #1: bs-0027R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), dot-blot

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: Unconjugated

Catalog #1: bs-20768R

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-75219c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-12201R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-80488C

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82470c-50-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11322R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Zebrafish

Conjugate: Unconjugated

Catalog #1: bs-70545R

Applications: WB, ICC

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bs-75529c-1g

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Antigen: bs-1988P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Menin\/Men1 Polyclonal Antibody, Unconjugated (bs-1988R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti Menin\/Men1 Polyclonal Antibody, Unconjugated (bs-1988R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1988R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-75663c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6056R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Mouse uterus lysates probed with MEPE Polyclonal Antibody, Unconjugated (bs-8689R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Spleen tissue lysates; Lane 4: Mouse Cerebellum tissue lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human MCF-7 cell lysates probed with MEPE Polyclonal Antibody, Unconjugated (bs-8689R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-8689R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-75635c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6539c-2mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-Merlin(Ser518) Polyclonal Antibody, Unconjugated (bs-3291R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Raw264.7 cell lysates probed with Phospho-NF2 (Ser518) Polyclonal Antibody, Unconjugated (bs-3291R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3291R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-72311M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72312M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72313M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-41398R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

293 cell lysates probed with MERTK (1C11) Monoclonal Antibody (bsm-52273R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with MERTK (1C11) Monoclonal Antibody (bsm-52273R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: HepG2 cell lysates; Lane 2: 293T cell lysates probed with MERTK Polyclonal Antibody, Unconjugated (bsm-52273R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n\r\n

Catalog #1: bsm-52273R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-18790R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-70156R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0548R

Applications: ELISA, IHC-P

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Mesothelin) Polyclonal Antibody, Unconjugated (bs-25346R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Mesothelin) Polyclonal Antibody, Unconjugated (bs-25346R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Mesothelin) Polyclonal Antibody, Unconjugated (bs-25346R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Mesothelin) Polyclonal Antibody, Unconjugated (bs-25346R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-25346R

Applications: IHC-P

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at\r\nroom temperature\r\nLysate: HeLa\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 69 kDa\r\nObserved MW: 40, 69 kDa
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Mesothelin) Monoclonal Antibody, Unconjugated (bsm-60005R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue:Human endometrium\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60005R
Tissue:Human cervical carcinoma\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60005R

Catalog #1: bsm-60005R

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

A549 probed with Mesothelin Polyclonal Antibody, Unconjugated (bs-0300R ) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Mesothelin Polyclonal Antibody, Unconjugated (bs-0300R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Hela lysates probed with Mesothelin Polyclonal Antibody, Unconjugated (bs-0300R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Mesothelin) Polyclonal Antibody, Unconjugated (bs-0300R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0300R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-C-Met\/Met\/HGFR Polyclonal Antibody, Unconjugated(bs-0668R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Formalin-fixed and paraffin embedded human gastric carcinoma labeled Anti-C-Met Polyclonal Antibody, Unconjugated (bs-0668R) at 1:100, followed by conjugation to the secondary antibody and DAB staining
Mouse nephrocytes probed with Rabbit Anti-Met (c Met) Polyclonal Antibody (bs-0668R) at 6ug for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-0668R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse Liver lysates probed with Met (c Met) Polyclonal Antibody, Unconjugated (bs-20721R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Liver tissue lysates probed with MET Polyclonal Antibody, Unconjugated (bs-20721R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-20721R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Antigen: bs-3271P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Lane 1: Rat Liver tissue lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human HepG2 cell lysates probed with Phospho-MET (Tyr1003) Polyclonal Antibody, Unconjugated (bs-3271R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3271R

Applications: WB, ELISA

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bs-1759R

Applications: ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-34227M

Applications: ICC

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431 treated with EGF lysates; Lane 2: Untreated A431 lysates probed with Met(C-Met) Polyclonal Antibody, Unconjugated (bsm-52370R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Flow cytometric analysis of HeLa lysate probed with Met(C-Met) (6C2) Monoclonal Antibody (bsm-52370R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with Met(C-Met) (6C2) Monoclonal Antibody (bsm-52370R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Met(C-Met) (6C2) Monoclonal Antibody (bsm-52370R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52370R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-70157R

Applications: WB, IHC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GRM2\/GLUR2 Polyclonal Antibody, Unconjugated (bs-1161R) at 1:300 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Human SH-SY5Y cell lysates probed with GRM2 \/Metabotropic glutamate receptor 2 Polyclonal Antibody, Unconjugated (bs-1161R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1161R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-mGluR4 Polyclonal Antibody, Unconjugated (bs-1894R) at 1:200 followed by conjugation to the secondary antibody
Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti GRM4\/mGluR4 Polyclonal Antibody, Unconjugated (bs-1894R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 102kD. Observed band size: 102kD.

Catalog #1: bs-1894R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70158R

Applications: WB, IHC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70159R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated