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Catalog #1: bsm-60330M

Applications: FCM, ICC

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human brain labeled with Anti- CXCL13\/BCA1 Polyclonal Antibody, Unconjugated (bs-2553R) at 1:200 followed by conjugation to the secondary antibody and DAB
Image was kindly submitted by Erica Pimenta from UMDNJ. Human breast tumor tissue stained with Rabbit Anti-CXCL13 Polyclonal Antibody (bs-2553R)
Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CXCL13 Polyclonal Antibody, Unconjugated (bs-2553R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining
Lane 1: Human K562 cell Lysates. Probed with CXCL13 polyclonal Antibody, unconjugated (bs-2553R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2553R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-4509R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1503R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2554R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-CXCL16 Polyclonal Antibody, Unconjugated (bs-1441R) followed by conjugation to the secondary antibody and DAB staining
Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-CXCL16 Polyclonal Antibody (bs-1441R) at 1:50 for two hours at room temperature (green channel).
BV2 (Human) lysates probed with CXCL16 Polyclonal Antibody, Unconjugated (bs-1441R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-1441R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8871R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-42278r-100ul

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-2549R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-41352R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-34043R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human spleen labeled with Anti-CXCL9\/MIG Polyclonal Antibody, Unconjugated (bs-2551R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse brain labeled with Anti-CXCL9\/MIG Polyclonal Antibody, Unconjugated (bs-2551R) followed by conjugation to the secondary antibody and DAB

Catalog #1: bs-2551R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colitis tissue labeled with Anti-CXCR1\/IL-8RA Polyclonal Antibody, Unconjugated (bs-1009R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat kidney lysates Lane 2: rat brain lysates probed with Anti CXCR1\/IL-8RA Polyclonal Antibody, Unconjugated (bs-1009R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 40kD. Observed band size: 40kD.
Lane 1: Human Jurkat cell Lysates; Lane 2: Human Raji cell Lysates. Probed with CXCR1 polyclonal Antibody, unconjugated (bs-1009R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lung tissue lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human Raji cell lysates probed with CXCR1 Polyclonal Antibody, Unconjugated (bs-1009R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1009R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-25519R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Bone lysates probed with CXCR2 (Ser347) Polyclonal Antibody, Unconjugated (bs-12257R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HL60 cell lysates; Lane 2: Mouse Skin lysates probed with CXCR2 (Ser347) Polyclonal Antibody, Unconjugated (bs-12257R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12257R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti CXC-R2 Polyclonal Antibody, Unconjugated (bs-4836R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Raw264.7 cell lysates; Lane 2: Human U937 cell lysates probed with CXCR2 Polyclonal Antibody, Unconjugated (bs-4836R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human THP-1 cell lysates; Lane 2: Human U937 cell lysates; Lane 3: Human HL60 cell lysates; Lane 4: Human HepG2 cell lysates probed with CXCR2 Polyclonal Antibody, Unconjugated (bs-4836R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4836R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat hepatoma labeled with Anti-CXCR2\/CD182 Polyclonal Antibody, Unconjugated (bs-1629R) followed by conjugation to the secondary antibody and DAB staining
Mouse bone lysates probed with Rabbit Anti-CXCR2\/CD182 Polyclonal Antibody, Unconjugated (bs-1629R) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

Catalog #1: bs-1629R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela Cells; Probed with CXCR3 (5F2) Monoclonal Antibody (bsm-54064R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with CXCR3 (5F2) Monoclonal Antibody (bsm-54064R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CXCR3 (5F2) Monoclonal Antibody (bsm-54064R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CXCR3 (5F2) Monoclonal Antibody (bsm-54064R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54064R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human nasal mucosa tissue labeled with Anti-CXCR3\/CD183 Polyclonal Antibody, Unconjugated (bs-0341R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse muscle lysates probed with CXCR3 Polyclonal Antibody, Unconjugated (bs-0341R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0341R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat exfoliated cells labeled with Anti-CXCR3 Polyclonal Antibody, Unconjugated (bs-2209R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HCCLM3 cells probed with Rabbit Anti-CXCR3 Polyclonal Antibody, Unconjugated (bs-2209R ) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Mouse Blood cell tissue lysates; Lane 2: Rat Blood cell tissue lysates probed with CXCR3 Polyclonal Antibody, Unconjugated (bs-2209R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2209R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-42318r-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-70442R

Applications: WB, IHC, ICC

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Rabbit Anti phospho-CXCR4(Ser339) Polyclonal Antibody, Unconjugated (bs-12256R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse Spleen cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXCR4 (Ser339) Polyclonal Antibody(bs-12256R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Lung lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse Uterus lysates; Lane 4: Mouse Lymph node lysates probed with CXCR4 Polyclonal Antibody, Unconjugated (bs-12256R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12256R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

L1 rat brain lysates L2 rat kidney lysates probed with Anti- CD184\/CXCR4 Polyclonal Antibody, Unconjugated (bs-1011R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 39kD.Observed band 39kD
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD184\/CXCR4 Polyclonal Antibody (bs-1011R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD184\/CXCR4 Polyclonal Antibody (bs-1011R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Anti-CXCR4 Polyclonal Antibody (bs-1011R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-1011R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-20318R

Applications: WB, ELISA, FCM

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Mouse bone lysates probed with CXCR4 Polyclonal Antibody, Unconjugated (bs-20317R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Blood cell lysates; Lane 2: Mouse Lymph node lysates; Lane 3: Rat Bone lysates; Lane 4: Rat Spleen lysates probed with CXCR4 Polyclonal Antibody, Unconjugated (bs-20317R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-20317R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Daudi Cells; Probed with CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Heart tissue incubated CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54319R

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse lymph node lysates probed with CXCR5 Polyclonal Antibody, Unconjugated (bs-3598R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Lane 1: Raji cell lysates; Lane 2: Mouse Lymph node lysates probed with CXCR5 Polyclonal Antibody, Unconjugated (bs-3598R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3598R

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60393M

Applications: WB, ICC

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of Jurkat cells with GPCR RDC1 (2G5) Monoclonal Antibody (bsm-52587R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
PC-3M cells Probed with CXCR7 (2G5) Monoclonal Antibody (bsm-52587R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-52587R

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CXCR7\/RDC1 Polyclonal Antibody, Unconjugated (bs-4897R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat kidney labeled with Anti-CXCR7\/RDC1 Polyclonal Antibody, Unconjugated (bs-4897R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-4897R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-9552R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CXorf36 Polyclonal Antibody, Unconjugated (bs-0488R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 mouse heart lysates probed with Anti CXorf36 Polyclonal Antibody, Unconjugated (bs-0488R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 20\/48kD. Observed band size:20kD.

Catalog #1: bs-0488R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0727R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-9550R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-14125R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-6890R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Mouse spinal cord lysates probed with CYB5R3 Polyclonal Antibody, Unconjugated (bs-12162R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CYB5R3) Polyclonal Antibody, Unconjugated (bs-12162R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human myocardium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CYB5R3) Polyclonal Antibody, Unconjugated (bs-12162R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Rat Liver tissue lysates; Lane 3: Rat Placenta tissue lysates probed with CYB5R3 Polyclonal Antibody, Unconjugated (bs-12162R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12162R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human laryngeal tissue labeled with Rabbit Anti-CYB5R4 Polyclonal Antibody, Unconjugated (bs-14133R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-CYB5R4 Polyclonal Antibody, Unconjugated (bs-14133R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse Testis lysates; Lane 2: Rat Testis lysates probed with CYB5R4 Polyclonal Antibody, Unconjugated (bs-14133R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-14133R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8297R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-22318R

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-5739R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Sheep

Conjugate: Unconjugated

Lane 1: PC-12 Cells; Lane 2: NIH\/3T3 Cells; Probed with Cyclin A2 (2C2) Monoclonal Antibody (bsm-52660R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Cyclin A2 (2C2) Monoclonal Antibody (bsm-52660R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Cyclin A2 (2C2) Monoclonal Antibody (bsm-52660R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Cyclin A2 (2C2) Monoclonal Antibody (bsm-52660R) at 1:300 in RH-35 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52660R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293; Lane 2: F9; Lane 3: PC12; Lane 4: Hela lysates probed with Cyclin A2 (2E9) Monoclonal Antibody (bsm-52043M) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human uterus \r\ntissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cyclin A2 (2E9) Monoclonal Antibody (bsm-52043M) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52043M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cyclin B1 (2E6) Monoclonal Antibody (bsm-52044R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 4\u2103\r\novernight\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: Jurkat, 2: HeLa, 3: HT-29\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 55 kDa\r\nObserved MW: 55 kDa

Catalog #1: bsm-52044R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclin B1 (Ser126) Polyclonal Antibody, Unconjugated (bs-3733R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human esophageal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclin B1 (Ser126) Polyclonal Antibody, Unconjugated (bs-3733R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclin B1 (Ser126) Polyclonal Antibody, Unconjugated (bs-3733R) at 1:200 overnight at 4\u00b0C, DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-Cyclin B1 (Ser126)) polyclonal Antibody, Unconjugated (bs-3733R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-3733R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3123R

Applications: WB, ELISA, FCM

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3122R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated