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Catalog #1: bs-6175R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-6175R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-6175R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-8307R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-8307R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-8307R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-5766R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-5766R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-5766R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RAB7 Polyclonal Antibody(bs-6703R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RAB7 Polyclonal Antibody, Unconjugated (bs-6703R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human HL-60 cell lysates probed with RAB7 Polyclonal Antibody, Unconjugated (bs-6703R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6703R

Applications: WB, ELISA, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-6703R-Biotin

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-6703R-HRP

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: HRP

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: HeLa, 2: A431, 3: MCF-7, 4: \r\nNIH\/3T3, 5: C6\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 23 kDa\r\nObserved MW: 23 kDa
Tissue: Human breast carcinoma\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60647R

Catalog #1: bsm-60647R

Applications: WB, IHC-P, IHC-F, ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60647R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bsm-60647R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RAB8A Polyclonal Antibody, Unconjugated (bs-6176R) at 1:500 overnight at 4\u00b0C, DAB staining.
NIH\/3T3 cell lysates probed with RAB8A Polyclonal Antibody, Unconjugated (bs-6176R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: Hela cell lysates probed with RAB8A Polyclonal Antibody, Unconjugated (bs-6176R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6176R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6168R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37\u00b0C for 30min; Antibody incubation with RAB9 Polyclonal Antibody, Unconjugated bs-6177R at 1:400 overnight at 4\u00b0C, , followed by a conjugated secondary antibody and DAB staining.
K562 cell lysates probed with RAB9 Polyclonal Antibody, Unconjugated (bs-6177R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell lysates probed with RAB9 Polyclonal Antibody, Unconjugated (bs-6177R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RAB9 Polyclonal Antibody, Unconjugated (bs-6177R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-6177R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-6177R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-6177R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-0312R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Avidin

Conjugate: Unconjugated

Catalog #1: bs-0311R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Biotin

Conjugate: Unconjugated

Catalog #1: bs-0326R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Bovine

Conjugate: Unconjugated

Catalog #1: bs-0326R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Bovine

Conjugate: Biotin

Catalog #1: bs-0326R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Bovine

Conjugate: HRP

Catalog #1: bs-0327R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Bovine

Conjugate: Unconjugated

Catalog #1: bs-0327R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Bovine

Conjugate: Biotin

Catalog #1: bs-0327R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Bovine

Conjugate: HRP

Catalog #1: bs-0310R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Unconjugated

Catalog #1: bs-0310R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Chicken

Conjugate: Biotin

Catalog #1: bs-0310R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Chicken

Conjugate: HRP

Catalog #1: bs-0314R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Unconjugated

Catalog #1: bs-0314R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Chicken

Conjugate: Biotin

Catalog #1: bs-0314R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Chicken

Conjugate: HRP

Catalog #1: bs-0303R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Dog

Conjugate: Unconjugated

Catalog #1: bs-0303R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Dog

Conjugate: Biotin

Catalog #1: bs-0303R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Dog

Conjugate: HRP

Catalog #1: bs-0294R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Goat

Conjugate: Unconjugated

Catalog #1: bs-0294R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: Biotin

Catalog #1: bs-0294R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: HRP

Catalog #1: bs-0370R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Goat

Conjugate: Unconjugated

Catalog #1: bs-0370R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: Biotin

Catalog #1: bs-0370R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: HRP

Catalog #1: bs-0358R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Guinea Pig

Conjugate: Unconjugated

Catalog #1: bs-0358R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Guinea Pig

Conjugate: Biotin

Catalog #1: bs-0358R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Guinea Pig

Conjugate: HRP

Catalog #1: bs-0375R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Guinea Pig

Conjugate: Unconjugated

Catalog #1: bs-0375R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Guinea Pig

Conjugate: Biotin