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Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin\/CD324 Polyclonal Antibody, Unconjugated(bs-10009R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Image provided by Independent Validation badge 29768.\\n\\nHistogram of MCF7 cells stained with Rabbit Anti-E-Cadherin Polyclonal Antibody (orange)(bs-10009R at 1:100), isotype control antibody (green), secondary antibody only (blue) and unstained (red)
Mouse splenocytes probed with Rabbit Anti-E cadherin Polyclonal Antibody, PE-Cy5.5 Conjugated (bs-10009R-PE-Cy5.5) at 1:50 for 40 minutes (green)compared to control cells (blue).
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-E cadherin Polyclonal Antibody (bs-10009R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-10009R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10009R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-10009R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded human gastric carcinoma tissue labeled with Anti-E-Selectin Polyclonal Antibody, Unconjugated(bs-1273R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma tissue labeled with Anti-E-Selectin Polyclonal Antibody, Unconjugated(bs-1273R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: DU145 cell lysates; Lane 2: LoVo cell lysates; probed with E-Selectin\/CD62E Polyclonal Antibody, Unconjugated (bs-1273R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1273R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-6835R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-6835R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-6835R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-2305R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-2305R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-2305R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Lane 1: Human Hela cell lysates; Lane 2: Human A673 cell lysates; Lane 3: Human Molt-4 cell lysates; Lane 4: Human U251cell lysates; Lane 5: Human HepG2 cell lysates probed with E2F1 (Ser332) Polyclonal Antibody, Unconjugated (bs-5306R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (phospho-E2F1 (Ser332) ) Polyclonal Antibody, Unconjugated (bs-5306R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-5306R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-5306R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-5306R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Lane 1: Mouse Skin lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse Large intestine lysates probed with E2F1 Polyclonal Antibody, Unconjugated (bs-5305R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with E2F1 (Ser337) Polyclonal Antibody(bs-5305R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Colon tissue lysates probed with phospho-E2F1 (Ser337) Polyclonal Antibody, Unconjugated (bs-5305R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-E2F1 (Ser337)) polyclonal Antibody, Unconjugated (bs-5305R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-5305R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-5305R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-5305R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-phospho-E2F1(Ser364)Polyclonal Antibody, Unconjugated (bs-4076R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human A673 cell lysates; Lane 3: Human MOLT-4 cell lysates probed with E2F1 Polyclonal Antibody, Unconjugated (bs-4076R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with E2F1 (Ser364) Polyclonal Antibody(bs-4076R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (phospho-E2F1 (Ser364)) Polyclonal Antibody, Unconjugated (bs-4076R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-4076R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4076R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-4076R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-E2F1 Polyclonal Antibody (bs-0599R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse skeleton lysates probed with E2F1 Polyclonal Antibody, Unconjugated (bs-0599R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with E2F1 Polyclonal Antibody(bs-0599R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0599R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with E2F1 Polyclonal Antibody, Unconjugated (bs-23185R) at 1:500 overnight at 4\u00b0C, DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human A673 cell lysates; Lane 3: Human MOLT-4 cell lysates; Lane 4: Human U251 cell lysates probed with E2F1 Polyclonal Antibody, Unconjugated (bs-23185R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (E2F1) polyclonal Antibody, Unconjugated (bs-23185R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (E2F1) polyclonal Antibody, Unconjugated (bs-23185R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-23185R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-23184R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-23185R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-23184R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-23185R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-23184R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-1722R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with E2F3 Polyclonal Antibody, Unconjugated (bs-21439R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with E2F3 Polyclonal Antibody, Unconjugated (bs-21439R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with E2F3 Polyclonal Antibody, Unconjugated (bs-21439R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-21439R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with E2F3 Polyclonal Antibody, Unconjugated (bs-21438R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with E2F3 Polyclonal Antibody, Unconjugated (bs-21438R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-21438R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1722R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-21439R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-21438R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1722R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-21439R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-21438R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti E2F5 Polyclonal Antibody, Unconjugated (bs-1734R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
RSC96 cells probed with E2F5 Polyclonal Antibody, Unconjugated (bs-1734R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1734R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-1750R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-EAAT1\/GLAST Polyclonal Antibody (bs-1003R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti EAAT1\/Glast Polyclonal Antibody, Unconjugated (bs-1003R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 56kD. Observed band size: 56kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (EAAT1) Polyclonal Antibody, Unconjugated (bs-1003R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (EAAT1) Polyclonal Antibody, Unconjugated (bs-1003R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1003R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-1003R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-1003R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-13046R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-13046R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-13046R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-14481R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-14481R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-14481R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-14482R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-14482R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin