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Catalog #1: bs-8148R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: C6, 2: HeLa Protein loading quantity: 20 \u03bcg Exposure time: 1: 60 s, 2: 30 s Predicted MW: 227 kDa Observed MW: 260 kDa
Tissue: Human breast Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60708R\r\n

Catalog #1: bsm-60708R

Applications: WB, IHC-P, IHC-F, IF, ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60708R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bsm-60708R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-8162R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-8162R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-8162R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-5250R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11226R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-11226R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-11226R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-5251R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Chicken

Conjugate: Unconjugated

Catalog #1: bs-5251R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Chicken

Conjugate: Biotin

Catalog #1: bs-5251R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Chicken

Conjugate: HRP

Catalog #1: bs-10410R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-10410R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-10410R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-4272R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

HepG2 cells probed with Rabbit Anti-CHI3L1 Polyclonal Antibody (bs-10215R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Secondary
Human HepG2 lysates probed with CHI3L1 Polyclonal Antibody, Unconjugated (bs-10215R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human HepG2 cell lysates; Lane 2: Recombinant human CHI3L1 Protein (bs-41221P) lysates probed with CHI3L1 Polyclonal Antibody, Unconjugated (bs-10215R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10215R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

L1 mouse embryo lysates L2 mouse liver lysates probed with Anti CGP-39\/YKL-40 Polyclonal Antibody, Unconjugated (bs-1093R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD
Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-CHI3L1 Polyclonal Antibody, Unconjugated (bs-1093R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CHI3L1) polyclonal Antibody, Unconjugated (bs-1093R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Lung tissue lysates; Lane 2: Rat Lung tissue lysates probed with CHI3L1 Polyclonal Antibody, Unconjugated (bs-1093R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1093R

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10215R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-10215R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-13900R

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CHIP Polyclonal Antibody, Unconjugated (bs-15405R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CHIP Polyclonal Antibody, Unconjugated (bs-15405R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-15405R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-15405R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-15405R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-13906R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-13906R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-13906R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-4000R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-4000R-Biotin

Applications: WB, ELISA, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-4000R-HRP

Applications: WB, ELISA, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-13908R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-13908R-Biotin

Applications: WB, ELISA, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-13908R-HRP

Applications: WB, ELISA, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-5260R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-5275R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-5275R-Biotin

Applications: WB, ELISA, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-5275R-HRP

Applications: WB, ELISA, IHC-F

Reactivity:

Conjugate: HRP

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-Phospho-CHK2 (Thr68) Polyclonal Antibody, Unconjugated (bs-3721R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
L1 rat brain lysates, L2 human colon carcinoma lysates probed with Anti- Phospho-CHK2(Thr68) Polyclonal Antibody, Unconjugated (bs-3721R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band size: 61kD Observed band size:61kD.\\n

Catalog #1: bs-3721R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bs-3721R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Biotin

Catalog #1: bs-3721R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: HRP

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti CHK2 Polyclonal Antibody, Unconjugated (bs-1391R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 human colon carcinoma lysates L2 human gastric carcinoma lysates probed with Anti CHK2 Polyclonal Antibody, Unconjugated (bs-1391R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 61kD. Observed band size: 61kD
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CHK2 Polyclonal Antibody(bs-1391R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1391R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-1391R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-1391R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-10307R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-10307R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-10307R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP