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Mouse lymph node probed with TNF alpha Polyclonal Antibody (bs-10802R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
THP-1 cells were treated with TPA (80 nM, overnight) and then treated with LPS (1 ug\/mL, 18 hr\/6 hr) and Brefeldin A (300 ng\/mL, last 3 hr of stimulation) .The cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TNF alpha Polyclonal Antibody(bs-10802R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Raw264.7 cells were treated with LPS (1 ug\/mL, 18 hr\/6 hr) and Brefeldin A (300 ng\/mL, last 3 hr of stimulation).The cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at at room temperature and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TNF alpha Polyclonal Antibody(bs-10802R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-10802R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-0078P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded human colitis tissue labeled with Anti-TNF-alpha Polyclonal Antibody, Unconjugated(bs-0077R) 1:300 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded human colitis tissue labeled with Anti-TNF-alpha Polyclonal Antibody, Unconjugated(bs-0077R) 1:300 followed by conjugation to the secondary antibody and DAB staining\\n
Human liver lysates probed with Anti TNF alpha Polyclonal Antibody, Unconjugated (bs-0078R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 17kD, 26kD. Observed band size:26kD.

Catalog #1: bs-0078R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: Unconjugated

THP-1 cells were treated with TPA (80 nM, overnight) and then treated with LPS (1 ug\/mL, 18 hr\/6 hr) and Brefeldin A (300 ng\/mL, last 3 hr of stimulation) .The cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TNF alpha Polyclonal Antibody(bs-41192R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Raw264.7 cells were treated with LPS (1 ug\/mL, 18 hr\/6 hr) and Brefeldin A (300 ng\/mL, last 3 hr of stimulation).The cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at at room temperature and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TNF alpha Polyclonal Antibody(bs-41192R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-41192R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0078R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: AbBy Fluor® 350

Catalog #1: bs-10802R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-2081R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Rabbit, Horse

Conjugate: AbBy Fluor® 350

Catalog #1: bs-41192R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0078R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: AbBy Fluor® 405

Catalog #1: bs-10802R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-2081R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Rabbit, Horse

Conjugate: AbBy Fluor® 405

Catalog #1: bs-41192R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0078R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: AbBy Fluor® 488

Catalog #1: bs-10802R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-2081R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Rabbit, Horse

Conjugate: AbBy Fluor® 488

Catalog #1: bs-41192R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0078R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: AbBy Fluor® 555

Catalog #1: bs-10802R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-2081R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Rabbit, Horse

Conjugate: AbBy Fluor® 555

Catalog #1: bs-41192R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0078R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: AbBy Fluor® 594

Catalog #1: bs-10802R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bs-2081R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Rabbit, Horse

Conjugate: AbBy Fluor® 594

Catalog #1: bs-41192R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0078R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: AbBy Fluor® 647

Catalog #1: bs-10802R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bs-2081R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Rabbit, Horse

Conjugate: AbBy Fluor® 647

Catalog #1: bs-41192R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0078R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: AbBy Fluor® 680

Catalog #1: bs-10802R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 680

Catalog #1: bs-2081R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Rabbit, Horse

Conjugate: AbBy Fluor® 680

Catalog #1: bs-41192R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0078R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: AbBy Fluor® 750

Catalog #1: bs-10802R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 750

Catalog #1: bs-2081R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Rabbit, Horse

Conjugate: AbBy Fluor® 750

Catalog #1: bs-41192R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0078R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: APC

Catalog #1: bs-10802R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-2081R-APC

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Rabbit, Horse

Conjugate: APC

Catalog #1: bs-41192R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bs-0078R-APC-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: APC-Cy5

Catalog #1: bs-10802R-APC-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5

Catalog #1: bs-2081R-APC-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Rabbit, Horse

Conjugate: APC-Cy5

Catalog #1: bs-41192R-APC-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy5

Catalog #1: bs-0078R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: APC-Cy5.5

Catalog #1: bs-10802R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-2081R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Rabbit, Horse

Conjugate: APC-Cy5.5

Catalog #1: bs-41192R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bs-0078R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: APC-Cy7