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Formalin-fixed and paraffin embedded rat brain labeled with Anti PARP (N-Terminus) Polyclonal Antibody, Unconjugated(bs-2138R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI
Lane 1: Rat Brain; Probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:1000 in 4˚C.
Human HeLa lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Human Raji lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-2138R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Mouse Testis lysates probed with PARP1 Polyclonal Antibody, Unconjugated (bs-20763R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human K562 cell lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human Hela cell lysates probed with PARP1 Polyclonal Antibody, Unconjugated (bs-20763R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-20763R) at 1:200 overnight at 4\u00b0C, DAB staining.
293T cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103 for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PARP1 Polyclonal Antibody(bs-20763R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-20763R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-20763R-BF350

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-20763R-BF405

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-20763R-BF488

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-20763R-BF555

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-20763R-BF594

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bs-20763R-BF647

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bs-20763R-BF680

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 680

Catalog #1: bs-20763R-BF750

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 750

Catalog #1: bs-20763R-APC

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-20763R-APC-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5

Catalog #1: bs-20763R-APC-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-20763R-APC-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-20763R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-20763R-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-20763R-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-20763R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-20763R-FITC

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-20763R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-20763R-PE-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy3

Catalog #1: bs-20763R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-20763R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-20763R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-20763R-PerCP

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-20763R-PerCP-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-20763R-PerCP-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bs-20763R-RBITC

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Flow cytometric analysis of SH-SY5Y cells with PARP2 (19A1) Monoclonal Antibody (bsm-54344R) at a 1:50 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).
Lane 1: SiHa Cells; Lane 2: Raji Cells; Probed with PARP2 (19A1) Monoclonal Antibody (bsm-54344R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-54344R

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse pancreas tissue labeled with Anti-PAX2 Polyclonal Antibody, Unconjugated (bs-1187R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-PAX2 Polyclonal Antibody, Unconjugated (bs-1187R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: mouse uterus lysates Lane 2: mouse brain lysates probed with Anti PAX2 Polyclonal Antibody, Unconjugated (bs-1187R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 46kD. Observed band size: 46kD.
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with PAX2 Antibody(bs-1187R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-1187R

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse embryo lysates Lane 2: human colon carcinoma lysates probed with Anti PAX3 Polyclonal Antibody, Unconjugated (bs-1097R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 53kD. Observed band size: 53kD
Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Anti-PAX3 Polyclonal Antibody, Unconjugated(bs-1097R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-PAX3 Polyclonal Antibody, Unconjugated(bs-1097R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebellum tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Rat Cerebellum tissue lysates; Lane 4: Rat Muscle tissue lysates probed with PAX3 Polyclonal Antibody, Unconjugated (bs-1097R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1097R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of Raji cells with PAX5 (4C1) Monoclonal Antibody (bsm-52735R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Daudi cells Probed with PAX5 (4C1) Monoclonal Antibody (bsm-52735R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with PAX5 (4C1) Monoclonal Antibody (bsm-52735R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with PAX5 (4C1) Monoclonal Antibody (bsm-52735R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52735R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Flow cytometric analysis of sh-sy5y cells with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Mouse eye lysate probed with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat eyes tissue incubated with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse eyes tissue incubated with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52682R

Applications: WB, FCM, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-11204P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti PAX6 Polyclonal Antibody, Unconjugated (bs-11204R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Pax6 Polyclonal Antibody, Unconjugated (bs-11204R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse embryo tissue fixed in 4% PFA and paraffin embedded\\nCitrate buffer (0.01M, pH 6.0) boiling bath for 15min; Anti-PAX6 Polyclonal Antibody, Unconjugated(bs-11204R) 1:200, overnight at 4\u00b0C; followed up by Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI was used to stain the cell nuclei.\\n

Catalog #1: bs-11204R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11204R-BF350

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-11204R-BF405

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-11204R-BF488

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-11204R-BF555

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-11204R-BF594

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bs-11204R-BF647

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bs-11204R-BF680

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 680

Catalog #1: bs-11204R-BF750

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 750

Catalog #1: bs-11204R-APC

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-11204R-APC-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5

Catalog #1: bs-11204R-APC-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-11204R-APC-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-11204R-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-11204R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5