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Catalog #1: bs-23303R-BF405

Applications: FCM

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 405

Catalog #1: bs-23303R-BF488

Applications: FCM

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 488

Catalog #1: bs-23303R-BF555

Applications: FCM

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 555

Catalog #1: bs-23303R-BF594

Applications: FCM

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 594

Catalog #1: bs-23303R-BF647

Applications: FCM

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 647

Catalog #1: bs-23303R-BF680

Applications: FCM

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 680

Catalog #1: bs-23303R-BF750

Applications: FCM

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 750

Catalog #1: bs-23303R-APC

Applications: FCM

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bs-23303R-APC-Cy5

Applications: FCM

Reactivity: Human, Mouse

Conjugate: APC-Cy5

Catalog #1: bs-23303R-APC-Cy5.5

Applications: FCM

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bs-23303R-APC-Cy7

Applications: FCM

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bs-23303R-Cy3

Applications: FCM

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-23303R-Cy5

Applications: FCM

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-23303R-Cy5.5

Applications: FCM

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bs-23303R-Cy7

Applications: FCM

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-23303R-FITC

Applications: FCM

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-23303R-PE

Applications: FCM

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bs-23303R-PE-Cy3

Applications: FCM

Reactivity: Human, Mouse

Conjugate: PE-Cy3

Catalog #1: bs-23303R-PE-Cy5

Applications: FCM

Reactivity: Human, Mouse

Conjugate: PE-Cy5

Catalog #1: bs-23303R-PE-Cy5.5

Applications: FCM

Reactivity: Human, Mouse

Conjugate: PE-Cy5.5

Catalog #1: bs-23303R-PE-Cy7

Applications: FCM

Reactivity: Human, Mouse

Conjugate: PE-Cy7

Catalog #1: bs-23303R-PerCP

Applications: FCM

Reactivity: Human, Mouse

Conjugate: PerCP

Catalog #1: bs-23303R-PerCP-Cy5.5

Applications: FCM

Reactivity: Human, Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bs-23303R-PerCP-Cy7

Applications: FCM

Reactivity: Human, Mouse

Conjugate: PerCP-Cy7

Catalog #1: bs-23303R-RBITC

Applications: FCM

Reactivity: Human, Mouse

Conjugate: RBITC

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody (bs-0465R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated (bs-0465R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).

Catalog #1: bs-0465R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: Unconjugated

U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NFKB p65 Polyclonal Antibody(bs-20355R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20355R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20355R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20355R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-20355R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Tissue\/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NFKB p65) polyclonal Antibody, Unconjugated (bs-23217R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human HeLa cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human U87MG cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human HepG2 cell lysates probed with NFKB p65 Polyclonal Antibody, Unconjugated (bs-23217R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-23217R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20160R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20160R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human Raji cell lysates probed with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20160R) at 1:200 dilution and 4ûC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37ûC.

Catalog #1: bs-20160R

Applications: WB, FCM, IHC-P, IHC-F, IF, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0465R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: AbBy Fluor® 350

Catalog #1: bs-20355R-BF350

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-23217R-BF350

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 350

Catalog #1: bs-20160R-BF350

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0465R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: AbBy Fluor® 405

Catalog #1: bs-20355R-BF405

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-23217R-BF405

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 405

Catalog #1: bs-20160R-BF405

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0465R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: AbBy Fluor® 488

Catalog #1: bs-20355R-BF488

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-23217R-BF488

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 488

Catalog #1: bs-20160R-BF488

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0465R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: AbBy Fluor® 555

Catalog #1: bs-20355R-BF555

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-23217R-BF555

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 555

Catalog #1: bs-20160R-BF555

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0465R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: AbBy Fluor® 594

Catalog #1: bs-20355R-BF594

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bs-23217R-BF594

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 594