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Catalog #1: bs-0725R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bs-2098R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-0122R-Cy7

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bs-0725R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-2098R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0122R-FITC

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-0725r-pe-100ul

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: PE

Catalog #1: bs-2098R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-0122R-PE

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: PE

Catalog #1: bs-0122R-PE-Cy3

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: PE-Cy3

Catalog #1: bs-2098R-PE-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy3

Catalog #1: bs-0122R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: PE-Cy5

Catalog #1: bs-2098R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-0725r-pe-cy5-100ul

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: PE-Cy5

Catalog #1: bs-0122R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-2098R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-0725r-pe-cy55-100ul

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-0122R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: PE-Cy7

Catalog #1: bs-2098R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-0725r-pe-cy7-100ul

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: PE-Cy7

Catalog #1: bs-0122R-PerCP

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: PerCP

Catalog #1: bs-2098R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-0725r-percp-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: PerCP

Catalog #1: bs-0122R-PerCP-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-2098R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0725r-percp-cy55-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0122R-PerCP-Cy7

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: PerCP-Cy7

Catalog #1: bs-2098R-PerCP-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bs-0122R-RBITC

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: RBITC

Catalog #1: bs-2098R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bs-0725r-rbitc-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: RBITC

MCF-7 cell lysates probed with Estrogen Receptor alpha(S118) (3A7 ) Monoclonal Antibody (bsm-52154R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Estrogen Receptor alpha(Ser118) (3A7) Monoclonal Antibody (bsm-52154R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
HUVEC cells were stained with Estrogen Receptor alpha(S118) (3A7) Monoclonal Antibody (bsm-52154R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
MCF7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Estrogen Receptor alpha(S118) (3A7 ) Monoclonal Antibody(bsm-52154R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-52154R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat ovary tissue labeled with Anti-ER beta Polyclonal Antibody, Unconjugated (bs-0116R), followed by conjugation to the secondary antibody and DAB staining
L1 mouse kidney lysates L2 mouse brain lysates probed with Anti ER-beta Polyclonal Antibody, Unconjugated (bs-0116R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000. Predicted band 58kD. Observed band size: 58kD
HeLa cells probed with Estrogen receptor beta Polyclonal Antibody, Unconjugated (bs-0116R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Mouse Testis lysates; Lane 2: Rat Testis lysates; Lane 3: Mouse Ovary lysates; Lane 4: Mouse Uterus lysates ; Lane 5: Rat Uterus lysates probed with Estrogen receptor beta Polyclonal Antibody, Unconjugated (bs-0116R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0116R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human brain tissue labeled with Rabbit Anti-ER-Beta Polyclonal Antibody, Unconjugated (bs-0255R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Mouse Ovary tissue lysates; Lane 4: Mouse Uterus tissue lysates; Lane 5: Rat Uterus tissue Lysates probed with Estrogen receptor beta Polyclonal Antibody, Unconjugated (bs-0255R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-0255R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0116R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0255r-bf350-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0116R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0255r-bf405-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0116R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0255r-bf488-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0116R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0255r-bf555-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0116R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0255r-bf594-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0116R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0255r-bf647-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0116R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0255r-bf680-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 680