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Catalog #1: bs-13614R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MYCL Polyclonal Antibody, Unconjugated (bs-24627R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MYCL Polyclonal Antibody, Unconjugated (bs-24627R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-24627R

Applications: ELISA, IHC-P

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-24627R-Biotin

Applications: ELISA, IHC-P

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-24627R-HRP

Applications: ELISA, IHC-P

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-4622R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Bacteria, Others

Conjugate: Unconjugated

Catalog #1: bs-4622R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Bacteria, Others

Conjugate: Biotin

Catalog #1: bs-4622R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Bacteria, Others

Conjugate: HRP

Catalog #1: bs-6460R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Bacteria

Conjugate: Unconjugated

Catalog #1: bsm-72321M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72322M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72323M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72324M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72325M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72326M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72327M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72328M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72329M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72330M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72332M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72334M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72333M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72331M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72335M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72336M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72337M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72338M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72339M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti-MyD88 Polyclonal Antibody, Unconjugated (bs-1047R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Molt-4 lysates probed with MyD88 Polyclonal Antibody, Unconjugated (bs-1047R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse Lung lysates probed with MyD88 Polyclonal Antibody, Unconjugated (bs-1047R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MyD88 Polyclonal Antibody, Unconjugated (bs-1047R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1047R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bs-1047R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Biotin

Catalog #1: bs-1047R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: HRP

Catalog #1: bs-11093R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-11093R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-11093R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Myelin Protein Zero Polyclonal Antibody, Unconjugated (bs-0337R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin-embedded rat lung labeled with Anti-Myelin Protein Zero Polyclonal Antibody, Unconjugated (bs-0337R)at 1:200 followed by conjugation to the secondary antibody and DAPI staining
Bone lysates probed with Myelin Protein Zero Polyclonal Antibody, Unconjugated (bs-0337R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Spinal cordl lysates; Lane 2: Rat Spinal cord lysates; Lane 3: Mouse Adipose lysates; Lane 4: Rat Adipose lysates probed with Myelin Protein Zero Polyclonal Antibody, Unconjugated (bs-0337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0337R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-0337R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Dog

Conjugate: Biotin

Catalog #1: bs-0337R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-19145R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-19145R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-19145R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-8200R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MYF5 Polyclonal Antibody, Unconjugated (bs-6936R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat tongue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MYF5 Polyclonal Antibody, Unconjugated (bs-6936R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Muscle tissue lysates; Lane 2: Mouse Tongue tissue lysates; Lane 3: Rat Muscle tissue lysates; Lane 4: Rat Tongue tissue lysates probed with MYF5 Polyclonal Antibody, Unconjugated (bs-6936R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6936R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6936R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-6936R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-15444R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-15444R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-15444R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

To determine the titer of the antibody, an ELISA was performed using a serial dilution MYH11 Polyclonal Antibody (bs-53091R). The plates were coated with the peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,900.
ChIP assays were performed using ME-1 cells, MYH11 Polyclonal Antibody (bs-53091R) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 \u03bcl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. The figure shows the relative occupancy, calculated as the ratio and control\/background for which the MYOG gene was used.
The IP\u2019d DNA from 6 ChIP\u2019s was pooled and subsequently analyzed on an Illumina HiSeq 2000. Library preparation, cluster generation, and sequencing were performed according to the manufacturer\u2019s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.

Catalog #1: bs-53091R

Applications: WB, ELISA, ChIP-seq

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51091R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated