MAP2K1 Monoclonal Antibody + Cell Lysate Kit
Applications
Reactivity
| Overview | |
| Catalog # | bsm-90040R |
| Product Name | MAP2K1 Monoclonal Antibody + Cell Lysate Kit |
| Applications | WB, FCM, IC |
| Specificity | Knockdown Validated |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | A synthesized peptide derived from human MEK1 |
| Immunogen Range | 1-40/393 |
| Clonality | Monoclonal |
| Clone # | 23GB2475 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Affinity Purified |
| Storage Buffer | Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide. |
| Storage Condition | -20 °C |
| Target | |
| Gene ID | 5604 |
| Swiss Prot | Q02750 |
| Synonyms | MAP2K1; Mitogen-Activated Protein Kinase Kinase 1; MEK1; Dual Specificity Mitogen-Activated Protein Kinase Kinase 1; MAPK/ERK Kinase 1; MAPKK1; PRKMK1; MKK1; ERK Activator Kinase 1; MAP Kinase Kinase 1; EC 2.7.12.2; MAPKK 1; MEK 1; Protein Kinase, Mitogen-Activated, Kinase 1 (MAP Kinase Kinase 1); CFC3; MEL |
| Background | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation. Cell Line: Hela Knockdown technology: shRNA Knockdown |
| Application Dilution | |
| WB | : 1:1000-1:5000 |
| FCM | FCM: 1:2000 |
| IC | IC: 1:1000 |
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