c-Src (N-terminal region) Antibody
Applications
Reactivity
Overview | |
Catalog # | bsm-70681M |
Product Name | c-Src (N-terminal region) Antibody |
Applications | WB, IP |
Specificity | This antibody detects a 60 kDa* protein corresponding to c-Src on SDS-PAGE immunoblots of mouse SYF cells transformed with c-Src and human A431 cells. |
Reactivity | Human, Mouse, Rat |
Specifications | |
Conjugation | Unconjugated |
Host | Mouse |
Source | Clone (M259) was generated from a recombinant protein containing amino acids in the N-terminal region of human c-Src. This sequence has high homology to similar regions in rat and mouse c-Src. |
Clonality | Monoclonal |
Clone # | M259 |
Isotype | IgG1 |
Purification | Purified by Protein A. |
Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
Target | |
Swiss Prot | P12931 |
Synonyms | Src |
Background | c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. The activity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor activation. Both Tyr-215 and Tyr-418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 downregulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity. |
Application Dilution | |
WB | 1:300-5000 |
IP | 1-2ug |