Overview |
bsm-70608M |
Phosphotyrosine Antibody |
WB, IP, ICC |
This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells. |
Human, Mouse, Rat, Chicken |
Specifications |
Unconjugated |
Mouse |
Clone M375 was generated from tyrosine phosphorylated proteins purified from pervanadate treated A431 cells. |
Monoclonal |
M375 |
IgG2b |
Purified by Protein A. |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
Target |
N/A |
Phosphotyrosine mAb |
Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity. |
Application Dilution |
WB |
1:300-5000 |
IP |
1-2ug |
ICC |
1:100-500 |