| Overview |
| bsm-70605M |
| Phosphoserine/threonine Antibody |
| WB, IP, ICC |
| The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA. Clone M380B was generated.from a phosphoserine synthetic peptide (coupled to carrier protein). |
| Human, Mouse, Rat |
| Specifications |
| Unconjugated |
| Mouse |
| PM3801 Phosphoserine/threonine is a mix of two clones: Clone M380A was generated from a phosphothreonine synthetic peptide (coupled to carrier protein) |
| pSer/Thr |
| Monoclonal |
| M380A/M380B |
| IgG1 |
| Lot dependent |
| Purified by Protein A. |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| N/A |
| Phosphoser/thr mAb |
| Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity. |
| Application Dilution |
| WB |
1:300-5000 |
| IP |
1-2ug |
| ICC |
1:100-500 |
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