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Cdk5 Antibody

Applications

  • WB
  • IP
  • ICC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-70420M
Product Name Cdk5 Antibody
Applications WB, IP, ICC
Specificity The antibody detects a 31 kDa* band corresponding to Cdk5 on SDS-PAGE immunoblots of human SYF and HeLa cells, and in mouse brain tissue. The antibody does not detect other Cdk family members, such as Cdk1 and Cdk2.
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Source Clones DC17+DC34 were generated from a recombinant full-length human Cdk5 sequence.
Clonality Monoclonal
Clone # DC17+DC34
Isotype IgG1
Purification Purified by Protein A.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot Q00535
Synonyms PSSALRE, TPKII
Background Cyclin-dependent kinases (Cdks) are a family of serine/threonine kinases that require association with regulatory subunits known as cyclins for activation. In addition, post-translational phosphorylation and dephosphorylation events regulate Cdk activity. Phosphorylation of Thr-160 in the T loop by Cdk-activating kinase (CAK) is an obligatory step in kinase activation. By contrast, phosphorylation of the Thr-14 and Tyr-15 residues by the Wee1 family of dual specificity kinases is inhibitory for the Cdks, and dephosphorylation of these residues by the Cdc25 family of phosphatases coincides with Cdk activation. Alternatively, Cdk5 appears to require different mechanisms for activation. This Cdk is activated through association with specific activators, including p35, p39, and p67. Cdk5 is primarily activated in neuronal cells, and only c-Abl kinase, rather than Wee family members, have been shown to phosphorylate Tyr-15 to regulate its activity.
Application Dilution
WB 1:300-5000
IP 1-2ug
ICC 1:100-500