Paxillin Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: A431, 3: U87-MG, 4: PC-3, 5: NIH-3T3\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 65 kDa\r\nObserved MW: 45-70 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: A431, 3: U87-MG, 4: PC-3, 5: NIH-3T3\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 65 kDa\r\nObserved MW: 45-70 kDa

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-60586R
Product Name	Paxillin Monoclonal Antibody
Applications	WB, FCM, ICC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Clonality	Monoclonal
Clone #	E2E8
Isotype	IgG
Concentration	1mg/ml
Purification	Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P49023
Application Dilution
WB	1:300-5000
FCM	1:20-100
ICC	1:100-500