RhoGAP Monoclonal Antibody

Siha cells were fixed,permeabilized and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RhoGAP Monoclonal Antibody(bsm-54725R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (red).

Siha cells were fixed,permeabilized and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RhoGAP Monoclonal Antibody(bsm-54725R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (red).

Applications

WB
FCM

Reactivity

Human

Overview
Catalog #	bsm-54725R
Product Name	RhoGAP Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within C-terminal Human RhoGAP.
Clonality	Monoclonal
Clone #	5D14
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	394
Swiss Prot	Q13017
Subcellular location	Cytoplasm, Cell membrane
Synonyms	ARHGAP 5 antibody, ARHGAP5 antibody, p190 B antibody, p190 RhoGAP antibody, Rho GTPase activating protein 5 antibody, RhoGAP 5 antibody, RhoGAP antibody, RhoGAP5 antibody
Background	Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Application Dilution
WB	1:300-5000
FCM	1:20-100

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Data Sheet

RhoGAP Monoclonal Antibody

$490

100ul

Qty

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