Neurocan Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Neurocan Monoclonal Antibody, Unconjugated (bsm-54723R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Neurocan Monoclonal Antibody, Unconjugated (bsm-54723R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Neurocan Monoclonal Antibody, Unconjugated (bsm-54723R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Neurocan Monoclonal Antibody, Unconjugated (bsm-54723R) at 1:200 for 30 minutes at room temperature, DAB staining.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54723R
Product Name	Neurocan Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within N-terminal human Neurocan.
Clonality	Monoclonal
Clone #	1H5
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Swiss Prot	O14594
Subcellular location	Secreted
Synonyms	Chondroitin sulfate proteoglycan 3 antibody, Cspg3 antibody, Ncan antibody, NCAN_HUMAN antibody, NEUR antibody, Neurocan core protein antibody, Neurocan proteoglycan antibody
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200