MUM1 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MUM1 Monoclonal Antibody, Unconjugated (bsm-54718R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MUM1 Monoclonal Antibody, Unconjugated (bsm-54718R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MUM1 Monoclonal Antibody, Unconjugated (bsm-54718R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MUM1 Monoclonal Antibody, Unconjugated (bsm-54718R) at 1:50 for 30 minutes at room temperature, DAB staining.

Applications

WB
IHC-P
IHC

Reactivity

Human

Overview
Catalog #	bsm-54718R
Product Name	MUM1 Monoclonal Antibody
Applications	WB, IHC-P, IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within N terminal human MUM1.
Clonality	Monoclonal
Clone #	6H0
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	3662
Swiss Prot	Q15306
Subcellular location	Nucleus
Synonyms	"Interferon regulatory factor 4 antibody, IRF 4 antibody, IRF-4 antibody, Irf4 antibody, IRF4_HUMAN antibody, LSIRF antibody, Lymphocyte specific interferon regulatory factor antibody, Lymphocyte specific IRF antibody, Lymphocyte-specific interferon regulatory factor antibody, Multiple myeloma oncogene 1 antibody, MUM 1 antibody, MUM1 antibody, NF EM5 antibody, NF-EM5 antibody, NFEM5 antibody, PU.1 interaction partner antibody, Sfpi1/PU.1 interaction partner antibody, Transcriptional activator PIP antibody"
Background	The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2010]
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC