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RNF40 (8D8) Monoclonal Antibody

Applications

  • WB
  • IHC-P
  • IP

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-54467R
Product Name RNF40 (8D8) Monoclonal Antibody
Applications WB, IHC-P, IP
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein within human RNF40 aa 200-400.
Clonality Monoclonal
Clone # 8D8
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 9810
Swiss Prot SwissProt:
Subcellular location Nucleus
Synonyms E3 ubiquitin-protein ligase BRE1B; BRE1-B; 95 kDa retinoblastoma-associated protein; RBP95; RING finger protein 40; RING-type E3 ubiquitin transferase BRE1B; KIAA0661
Background Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IP 1-2ug