Histone H2AX (9C9) Monoclonal Antibody

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:250, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:250, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:250, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:250, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:250, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:250, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Bratin tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:250, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Bratin tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:250, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody (green). The nuclear counterstain is DAPI (blue).$

Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody (green). The nuclear counterstain is DAPI (blue).

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody (green). The nuclear counterstain is DAPI (blue).$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody (green). The nuclear counterstain is DAPI (blue).

$Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody (green). The nuclear counterstain is DAPI (blue).$

Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody (green). The nuclear counterstain is DAPI (blue).

$Lane 1: MCF-7 Cells; Probed with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: MCF-7 Cells; Probed with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54151R
Product Name	Histone H2AX (9C9) Monoclonal Antibody
Applications	WB, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within human Histone H2A.X aa 1-50
Clonality	Monoclonal
Clone #	9C9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	3014
Swiss Prot	P16104
Synonyms	Histone H2A.X; Histone H2AX; H2a/x; H2AX; H2AFX.; H2A histone family member X.
Background	Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200