KMT5A (2A1) Monoclonal Antibody

$Lane 1: Mouse Kidney; Lane 2: Mouse Liver; Lane 3: 293T Cells; Lane 4: 293 Cells; Probed with KMT5A (2A1) Monoclonal Antibody (bsm-54112R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Kidney; Lane 2: Mouse Liver; Lane 3: 293T Cells; Lane 4: 293 Cells; Probed with KMT5A (2A1) Monoclonal Antibody (bsm-54112R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Flow cytometric analysis of 293T cells with KMT5A (2A1) Monoclonal Antibody (bsm-54112R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54112R
Product Name	KMT5A (2A1) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within human KMT5A 50-100
Clonality	Monoclonal
Clone #	2A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	387893
Swiss Prot	Q9NQR1
Synonyms	N-lysine methyltransferase KMT5A, H4-K20-HMTase KMT5A, Histone-lysine N-methyltransferase KMT5A, Lysine N-methyltransferase 5A, Lysine-specific methylase 5A, PR/SET domain-containing protein 07, SET domain-containing protein 8, PR-Set7, PR/SET07, KMT5A, PRSET7, SET07, SET8, SETD8
Background	Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.
Application Dilution
WB	1:300-5000
FCM	1:20-100