RBCK1 Monoclonal Antibody

Lane 1: Jurkat cell lysates; Lane 2: 293 cell lysates; Lane 3: A375 cell lysates probed with RBCK1 Monoclonal Antibody, Unconjugated (bsm-51685M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Predicted Reactivity

Human

Overview
Catalog #	bsm-51685M
Product Name	RBCK1 Monoclonal Antibody
Applications	WB
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human RBCK1 (UBCE7IP3).
Clonality	Monoclonal
Clone #	R5E2
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10616
Swiss Prot	Q9BYM8
Subcellular location	Nucleus
Synonyms	C20orf18; HOIL1_HUMAN; RanBP-type and C3HC4-type zinc finger-containing protein 1; HBV-associated factor 4; Heme-oxidized IRP2 ubiquitin ligase 1; HOIL-1; Hepatitis B virus X-associated protein 4; RING finger protein 54; RING-type E3 ubiquitin transferase HOIL-1; Ubiquitin-conjugating enzyme 7-interacting protein 3; RNF54; UBCE7IP3; XAP3; XAP4.
Background	Acts as an E3 ubiquitin-protein ligase, or as part of an E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteoasome. Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates. LUBAC conjugates linear polyubiquitin to IKBKG at 'Lys-285' and 'Lys-309' and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
Application Dilution
WB	=1:500-2000Ôºå