PDXK Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PDXK Monoclonal Antibody, Unconjugated (bsm-51665M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PDXK Monoclonal Antibody, Unconjugated (bsm-51665M) at 1:25 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P

Predicted Reactivity

Human

Overview
Catalog #	bsm-51665M
Product Name	PDXK Monoclonal Antibody
Applications	WB, IHC-P
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human PDXK between 1-312 amino acids.
Clonality	Monoclonal
Clone #	H8F01
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8566
Swiss Prot	O00764
Subcellular location	Cytoplasm
Synonyms	PDXK.1; PDXK_HUMAN; PKH; PNK; Pyridoxal kinase; Pyridoxamine kinase; Pyridoxine kinase; Vitamin B6 kinase.
Background	Required for synthesis of pyridoxal-5-phosphate from vitamin B6.
Application Dilution
WB	=1:500-4000ÔºåIHC-P=1:25ÔºåIHC-F=1:25Ôºå
IHC-P	1:200-400