RANBP9 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RANBP9 Monoclonal Antibody, Unconjugated (bsm-51664M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RANBP9 Monoclonal Antibody, Unconjugated (bsm-51664M) at 1:25 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P

Predicted Reactivity

Human

Overview
Catalog #	bsm-51664M
Product Name	RANBP9 Monoclonal Antibody
Applications	WB, IHC-P
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human RANBP9 between 1-388 amino acids.
Clonality	Monoclonal
Clone #	B5F6
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10048
Swiss Prot	Q96S59
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	RANB9_HUMAN; Ran-binding protein 9; RanBP9; BPM-L; BPM90; Ran-binding protein M; RanBPM; RanBP7.
Background	May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.
Application Dilution
WB	=1:500-4000ÔºåIHC-P=1:25ÔºåIHC-F=1:25Ôºå
IHC-P	1:200-400