EIF2AK2 Monoclonal Antibody

$Lane 1: A431 cell lysates; Lane 2: HepG2 cell lysates; Lane 3: SH-SY5Y cell lysates probed with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-51601M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: A431 cell lysates; Lane 2: HepG2 cell lysates; Lane 3: SH-SY5Y cell lysates probed with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-51601M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Predicted Reactivity

Human

Overview
Catalog #	bsm-51601M
Product Name	EIF2AK2 Monoclonal Antibody
Applications	WB, IF, ICC
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human EIF2AK2.
Clonality	Monoclonal
Clone #	R5D3
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5610
Swiss Prot	P19525
Subcellular location	Cytoplasm
Synonyms	double-stranded RNA-dependent Protein Kinase; interferon-induced, double-stranded RNA-activated protein kinase isoform a; protein kinase, interferon-inducible double stranded RNA dependent; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; p68 kinase; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; protein kinase RNA-activated; interferon-inducible RNA-dependent protein kinase; EIF2AK1; MGC126524; PKR; PRKRv; E2AK2_HUMAN.
Background	Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
Application Dilution
WB	=1:500-2000ÔºåICC=1:25ÔºåIF=1:25Ôºå
IF
ICC	1:100-500