FABP4 (5C1) Monoclonal Antibody

$Lane 1: Human placenta lysates; Lane 2: 3T3-L1 Cell lysates; probed with FABP4 (1105CT1.1.1) Monoclonal Antibody, unconjugated (bsm-51247M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Human placenta lysates; Lane 2: 3T3-L1 Cell lysates; probed with FABP4 (1105CT1.1.1) Monoclonal Antibody, unconjugated (bsm-51247M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded Human lungtissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ANTIBODY Monoclonal Antibody (bsm-51247M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human lungtissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ANTIBODY Monoclonal Antibody (bsm-51247M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

$Lane 1: Mouse Adipose tissue lysates; Lane 2: Mouse Breast tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Thymus tissue lysates probed with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Adipose tissue lysates; Lane 2: Mouse Breast tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Thymus tissue lysates probed with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bsm-51247M
Product Name	FABP4 (5C1) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Purified His-tagged FABP4 protein was used to produced this monoclonal antibody.
Clonality	Monoclonal
Clone #	5C1
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2167
Swiss Prot	P15090
Synonyms	aP2; ALBP; AFABP; A-FABP; HEL-S-104; Fatty acid-binding protein, adipocyte; Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; Fatty acid-binding protein 4; FABP4
Background	Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus (By similarity).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400