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ALDH1A1 (2C2) Monoclonal Antibody

Applications

  • WB
  • FCM
  • IHC-P
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-51032M
Product Name ALDH1A1 (2C2) Monoclonal Antibody
Applications WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source This ALDH1A1 monoclonal antibody is generated from mouse immunized with ALDH1A1 recombinant protein.
Clonality Monoclonal
Clone # 2C2
Isotype IgG1
Concentration 0.5ug/ul
Purification Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 216
Swiss Prot P00352
Subcellular location Cytoplasm
Synonyms ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e; Retinal dehydrogenase 1; RALDH 1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic; ALDH1A1.
Background ALDH1A1 belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. ALDH1A1 is the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. ALDH1A1 binds free retinal and cellular retinol-binding protein-bound retinal. ALDH1A1 can convert/oxidize retinaldehyde to retinoic acid. ALDH1A1 may have a broader specificity and oxidize other aldehydes in vivo.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200