Strep-Tag II (Mix-mA™) Monoclonal Antibody

$Strep-Tagged Fusion Protein Overexpression E.coli Lysates (2\/4ug,Cat#: bs-41403P) probed with Strep-Tag II Monoclonal Antibody, Unconjugated (bsm-33166M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Strep-Tagged Fusion Protein Overexpression E.coli Lysates (2\/4ug,Cat#: bs-41403P) probed with Strep-Tag II Monoclonal Antibody, Unconjugated (bsm-33166M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Tags
Others

Overview
Catalog #	bsm-33166M
Product Name	Strep-Tag II (Mix-mA™) Monoclonal Antibody
Applications	WB, ELISA
Reactivity	Tags, Others
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide of Strep-Tag
Clonality	Monoclonal
Clone #	Mix-mA™
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms	Strep; Strep Tag II; Strep.Tag; Strep-Tag-II; Strep-Tag-II.
Background	The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000