Mouse Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) ELISA Kit
Principle of the Assay
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SAT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain SAT1, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
For Use with serum, plasma, and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.
Target Information
Enzyme which catalyzes the acetylation of polyamines (PubMed:18690703). Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine (By similarity). This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines (By similarity). Also involved in the regulation of polyamine transport out of cells (By similarity). Also acts on 1,3-diaminopropane and 1,5-diaminopentane (By similarity).
GENE ID | 20229 |
SWISS PROT | P48026 |
SYNONYMS |
DC21; KFSD; SAT; SSAT; SSAT-1; Diamine Acetyltransferase 1; Polyamine N-acetyltransferase 1; Putrescine acetyltransferase |
Materials Supplied
Kit Components | 96 Wells Quantity/Size |
---|---|
Pre-coated, ready-to-use 96-well strip plate | 1 plate |
Plate sealer for 96 wells | 2 |
Standard |
2 tubes |
Diluent buffer | 1 bottle |
Detection Reagent A | 1 bottle |
Detection Reagent B | 1 bottle |
TMB Substrate | 1 tube |
Stop Solution | 1 tube |
Wash Buffer (30 ℅ concentrate) | 1 tube |
Product data sheet | 1 copy |
Storage
Storage | The TMB Substrate, Wash Buffer (30X concentrate), and the Stop Solution should be stored at 4°C upon receipt, while the other items should be stored at -20°C. |
Performance Characteristics
REPEATABILITY |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level SAT1 were tested 20 times on one plate, respectively. |
SENSITIVITY | The minimum detectable dose was 0.117ng/mL. |
ASSAY RANGE | 0.312-20ng/mL |
SPECIFICITY | This assay has high sensitivity and excellent specificity for the detection of SAT1. No significant cross-reactivity or interference between SAT1 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between SAT1 and all analogs, therefore, cross reactivity may still exist. |