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Biotin-(5-fluorescein)-conjugate

Overview
Catalog # bs-75917c-1mg
Product Name Biotin-(5-fluorescein)-conjugate
Specifications
Storage Buffer Solid
Storage Condition Stable for 2 years after receipt when stored at -20°C.
Target
Product Information CAS Number: 957494-27-8

Molecular formula: C33H32N4O8S

Molecular weight: 644.7

Purity: >90% (HPLC)

Appearance: Yellow solid.

Solubility: Soluble in DMSO.

InChiKey: DFUFXKZUEOKPSD-LFERIPGTSA-N

SMILES: O=C1C=C2OC3=CC(O)=CC=C3C(C4=C(C(O)=O)C=C(C(NCCNC(CCCC[C@H]5[C@](NC(N6)=O)([H])[C@]6([H])CS5)=O)=O)C=C4)=C2C=C1
Description Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: lambdaex 490nm; lambdaem 524nm in 0.1M Tris pH8.0.