Rhod 2-AM

Overview
Catalog #	bs-75817c-1mg
Product Name	Rhod 2-AM
Specifications
Storage Buffer	Solid
Storage Condition	Stable for 2 years after receipt when stored at -20°C.
Target
Product Information	CAS Number: 145037-81-6 Molecular formula: C52H59BrN4O19 Molecular weight: 1123.96 Purity: >80% (HPLC) Appearance: Red-purple solid. Solubility: Soluble in DMSO, DMF, methanol or chloroform. InChiKey: RWXWZOWDWYQKBK-UHFFFAOYSA-M SMILES: O=C(OCOC(C)=O)CN(CC(OCOC(C)=O)=O)C1=C(C=C(C=C1)C2=C3C=C/C(C=C3OC4=CC(N(C)C)=CC=C24)=[N+](C)/C)OCCOC5=C(C=CC(C)=C5)N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O.[Br-]
Description	Long-wavelength cell-permeant Ca2+ indicator. Rhod-2/AM is an acetoxymethyl ester derivative of Rhod 2 that can be easily loaded into cells via incubation and used for quantifying intracellular Ca2+ concentration allowing non-invasive measurement of calcium ions in live cells. This fluorogenic probe for intracellular Ca2+ operates in the visible spectrum. Rhod-2/AM itself does not bind Ca2+, but it is readily hydrolyzed to Rhod-2 by endogenous esterases once the dye enters cells. Rhod-2 is a valuable alternative to Fluo-3 to investigate changes in intracellular free Ca2+ concentrations, especially in cells and tissues that have high levels of autofluorescence. Rhod-2 has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, whose excitation and emission maxima make it suitable for use with argon and krypton laser. Its signal intensity is the strongest of all the calcium probes. Spectral Data: lambdaex 550nm, lambdaem 578nm in methanol; lambdaex 549nm, lambdaem 578nm in DMSO; lambdaex 550nm, lambdaem 570nm in Chloroform:Methanol (90:10).