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X-Gal

Overview
Catalog # bs-75002c-50mg
Product Name X-Gal
Specifications
Storage Buffer Powder
Storage Condition Stable for 3 years after receipt when stored at -20°C.
Target
Product Information CAS Number: 7240-90-6

Molecular formula: C14H15BrClNO6

Molecular weight: 408.6

Purity: >99% (HPLC)

Appearance: White to off-white powder. Clear, colourless to light yellow in solution.

Solubility: Soluble in dimethyl formamide.

InChiKey: OPIFSICVWOWJMJ-LMNFNBGQSA-N

SMILES: OCC1O[C@@H](OC2=CNC3=C2C(Cl)=C(Br)C=C3)C(O)[C@H](O)[C@H]1O
Description Molecular biology reagent. Chromogenic substrate for beta-galactosidase. Used in gene cloning together with IPTG (Prod. No. bs-75001C) to indicate whether a cell expresses the beta-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening. Distinguishes between recombinant and non-recombinant plasmids carrying the beta-galactosidase gene. Used in two-hybrid analysis. X-Gal is used to determine E. coli and coliform content in drinking water samples.