Overview |
bs-70607R |
Phosphothreonine Antibody |
WB, IP, ICC |
This antibody was cross-adsorbed to unphosphorylated peptide then Affinity purification using phospho-threonine peptide (without carrier). The antibody detects many serine or threonine phosphorylated proteins by western blot and ELISA. |
Human, Mouse, Rat |
Specifications |
Unconjugated |
Rabbit |
Phosphothreonine synthetic peptides coupled to carrier protein. |
pThr |
Polyclonal |
IgG |
Lot dependent |
Antigen Affinity purification |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
Target |
N/A |
Phosphoser/thr mAb |
Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity |
Application Dilution |
WB |
1:300-5000 |
IP |
1-2ug |
ICC |
1:100-500 |