HTH-01-015

Overview
Catalog #	bs-6435c-10mg-solid
Product Name	HTH-01-015
Specifications
Storage Buffer	Powder
Storage Condition	Store in dry, dark place at -20C for 1 year.
Target
Product Information	Molecular Weight: 468.55 Formula: C26 H28 N8 O CAS Number: 1613724-42-7 InChi Key: CHSDJDLAKKAWCI-UHFFFAOYSA-N InChi: InChI=1S/C26H28N8O/c1-16-23-24(31-26(29-16)30-19-14-28-34(15-19)20-8-10-27-11-9-20)32(2)22-13-18-7-5-4-6-17(18)12-21(22)25(35)33(23)3/h4-7,12-15,20,27H,8-11H2,1-3H3,(H,29,30,31) Smiles: CC1=NC(NC2=CN(N=C2)C2CCNCC2)=NC2=C1N(C)C(=O)C1C=C3C=CC=CC3=CC=1N2C Purity: 98.0 Solubility: Soluble in DMSO Appearance: Solid Power Shelf Life: 1.0 years
Description	HTH-01-015 is a potent and selective inhibitor of NUAK1 (IC50 = 100 nM) and does not affect the activity of a panel of 139 other kinases, including additional AMPK family members. The administration of HTH-01-015 to MEFs (mouse embryonic fibroblasts) significantly inhibits migration in a wound-healing assay to a similar extent as NUAK1-knockout. HTH-01-015 also inhibit proliferation of MEFs to the same extent as NUAK1 knockout and U2OS cells to the same extent as NUAK1 shRNA knockdown. HTH-01-015 impaired the invasive potential of U2OS cells in a 3D cell invasion assay to the same extent as NUAK1 knockdown. HTH-01-015 will serve as useful chemical probes to delineate the biological roles of the NUAK kinases.