Overview |
bs-55084R |
GIT1 Polyclonal Antibody |
WB, IF(ICC), IF |
Human, Mouse, Rat |
Specifications |
Unconjugated |
Rabbit |
Recombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2). |
Polyclonal |
IgG |
1ug/ul |
Purified by Protein A. |
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Store at -20°C for 12 months. |
Target |
28964 |
Q9Y2X7 |
Cytoplasm, Cell membrane |
ARF GAP GIT1; ARF GTPase activating protein GIT1; CAT 1; Cat1; Cool associated and tyrosine phosphorylated protein 1; G protein coupled receptor kinase interactor 1; GRK interacting protein 1. |
Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist (1,2). G protein-coupled receptor kinases (GRKs) are activated by activated G protein-coupled receptors, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade (3,4). GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, which are involved in vesicular trafficking (5,6). GIT1 overexpression results in reduced internalization and resensitization of b2-adrenergic receptor, thus reducing b2-adrenergic receptor signaling (5). |
Application Dilution |
WB |
1:300-5000 |
IF(ICC) |
1:50-200 |
IF |
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