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CD1d1 Polyclonal Antibody

Lane 1: mouse liver lysates Lane 2: mouse thymus lysates probed with Anti CD1d1 Polyclonal Antibody, Unconjugated (bs-4961R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Observed band size: 34kD
Lane 1: mouse liver lysates Lane 2: mouse thymus lysates probed with Anti CD1d1 Polyclonal Antibody, Unconjugated (bs-4961R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Observed band size: 34kD
Mouse splenocytes probed with CD1d1 Polyclonal Antibody, Unconjugated (bs-4961R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse splenocytes probed with CD1d1 Polyclonal Antibody, Unconjugated (bs-4961R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P

Reactivity

  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-4961R
Product Name CD1d1 Polyclonal Antibody
Applications WB, ELISA, FCM, IHC-P
Reactivity Mouse
Predicted Reactivity Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse CD1d1
Immunogen Range 165-270/336
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 12479
Swiss Prot P11609
Subcellular location Cytoplasm, Cell membrane, Extracellular matrix
Synonyms Cd1a; Cd1d; CD1.1; Ly-38; AI747460; Antigen-presenting glycoprotein CD1d1; CD1d.1; Cd1d1
Background Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

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