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AMPK gamma 3/PRKAG3 Polyclonal Antibody, PE Conjugated

Applications

  • WB

Reactivity

  • Human
  • Others

Predicted Reactivity

  • Mouse
  • Rat
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-3968R-PE
Product Name AMPK gamma 3/PRKAG3 Polyclonal Antibody, PE Conjugated
Applications WB
Reactivity Human, Others
Predicted Reactivity Mouse, Rat, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human AMPK gamma 3
Immunogen Range 151-250/489
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 53632
Swiss Prot Q9UGI9
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms AMPKG3; 5'-AMP-activated protein kinase subunit gamma-3; AMPK gamma3; AMPK subunit gamma-3; PRKAG3
Background AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
Application Dilution
WB 1:300-5000