GGNBP2 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

ELISA
IHC-P

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Cow
Sheep
Horse
Pig
Rabbit

Overview
Catalog #	bs-25129R
Product Name	GGNBP2 Polyclonal Antibody
Applications	ELISA, IHC-P
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Cow, Sheep, Horse, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GGNBP2
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	79893
Swiss Prot	Q9H3C7
Synonyms	DIF-3; DIF3; FLJ21230; FLJ22561; Gametogenetin-binding protein 2; GGNB2_HUMAN; Ggnbp2; Laryngeal carcinoma-related protein 1; LCRG1; LZK1; Protein ZNF403; ZFP403; ZNF403.
Background	LCRG1 is a 697 amino acid protein that may be involved in spermatogenesis. LCRG1 interacts with gametogenetin, a protein primarily expressed in testis and ovary. Localized in the cytoplasm, LCRG associates with vesicular structures. LCRG1 is expressed in brain, placenta, lung, liver and kidney, with highest levels found in heart, pancreas and skeletal muscle. LCRG1 is strongly down-regulated in 40% of primary laryngeal carcinoma and in 6 of 10 various cancer cell lines. Phosphorylated on Serine 360, LCRG1 exists as three isoforms produced by alternative splicing events.
Application Dilution
ELISA	1:5000-10000
IHC-P	1:100-500