New to Bioss? Enjoy 35% of your first order. Use code "FirstOrder35" - Offer valid for new U.S. Customers on direct orders only

Lck, AbBy Fluor® 350 Conjugated

Applications

  • WB

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Pig
  • Rabbit
Overview
Catalog # bs-24944R-BF350
Product Name Lck, AbBy Fluor® 350 Conjugated
Applications WB
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Pig, Rabbit
Specifications
Conjugation AbBy Fluor® 350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Lck
Immunogen Range 21-120/509
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3932
Swiss Prot P06239
Subcellular location Cytoplasm, Cell membrane
Synonyms p56-LCK; p56-LCK; Lymphocyte cell-specific protein-tyrosine kinase; LSK; T cell-specific protein-tyrosine kinase; Lck p56; Lymphocyte Specific Protein Tyrosine Kinase; Membrane associated protein tyrosine kinase; Oncogene lck; P56 LCK; p56(LSTRA) protein tyrosine kinase; p56lck; pp58 lck; pp58lck; Proto oncogene tyrosine protein kinase LCK; Protooncogene tyrosine protein kinase LCK; T cell specific protein tyrosine kinase; T lymphocyte specific protein tyrosine kinase p56lck; YT 16; YT16; LCK_HUMAN.
Background Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.
Application Dilution
WB 1:300-5000