Fos B (Ser27) Polyclonal Antibody

Raji cell lysates probed with phospho-Fos B (Ser27) Polyclonal Antibody, Unconjugated (bs-13198R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Siha cell lysates probed with phospho-Fos B (Ser27) Polyclonal Antibody, Unconjugated (bs-13198R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Sheep
Pig
Horse
Guinea Pig

Overview
Catalog #	bs-13198R
Product Name	Fos B (Ser27) Polyclonal Antibody
Applications	WB, ELISA, FCM
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human Fos B around the phosphorylation site of Ser27
Modification Site	Ser27
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2354
Subcellular location	Nucleus
Synonyms	Fos B phospho S27; p-Fos B phospho S27; p-FosB phospho S27; FBJ murine osteosarcoma viral oncogene homolog B; FOS B; FOS-B; FBJ osteosarcoma oncogene B; FosB; G0/G1 switch regulatory protein 3; G0S3; GOS3; GOSB; MGC42291; Oncogene FOS B; Protein fosB; fosB; G0/G1 switch regulatory protein 3; G0S3; GOS 3; GOS B; GOSB; Oncogene FOS B; Oncogene FOSB; Protein fos B; DKFZp686C0818; FOSB_HUMAN.
Background	The v-Fos oncogene was initially identified as the transforming gene of two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. The cellular homolog, c-Fos, encodes a nuclear phosphoprotein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers which bind to specific DNA TPA response elements (TREs), c-Fos proteins are only active as heterodimers with members of the Jun gene family. Murine Fos B encodes a nuclear protein of 338 amino acids which has 70% homology with c-Fos, exhibits similar kinetics of expression as c-Fos and forms heterodimers with both c-Jun and Jun B which bind to TRE DNA response elements. Functional homologs of c-Fos and Fos B include Fra-1 and Fra-2 genes.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100